培养中的肝细胞分化。酪氨酸转氨酶的出现。
Hepatocyte differentiation in culture. Appearance of tyrosine aminotransferase.
作者信息
Yeoh G C, Bennett F A, Oliver I T
出版信息
Biochem J. 1979 Apr 15;180(1):153-60. doi: 10.1042/bj1800153.
Abstract
Liver of rat foetuses from 14 to 19 days of gestation and cultured hepatocytes derived from foetuses of 14 or 15 days gestation show a limited capacity to transaminate tyrosine. This low tyrosine transamination activity can be ascribed to aspartate aminotransferase. Definitive tyrosine aminotransferase can be demonstrated in 1-day-old cultures of hepatocytes taken from 19-day foetuses, but not from 15-day foetuses. However, after 3 days of culture hepatocytes from 15-day foetuses are able to synthesize tyrosine aminotransferase. Induction studies reveal that dexamethasone is capable of increasing tyrosine aminotransferase activity once it is detectable in culture.
摘要
妊娠14至19天的大鼠胎儿肝脏以及源自妊娠14或15天胎儿的培养肝细胞，转氨作用于酪氨酸的能力有限。这种低酪氨酸转氨活性可归因于天冬氨酸转氨酶。从19天胎儿的肝细胞1日龄培养物中可证实有明确的酪氨酸转氨酶，但从15天胎儿的肝细胞中则无法证实。然而，培养3天后，15天胎儿的肝细胞能够合成酪氨酸转氨酶。诱导研究表明，一旦在培养物中可检测到，地塞米松就能够增加酪氨酸转氨酶活性。
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