Cake M H, Yeoh G C, Oliver I T
Biochem J. 1981 Aug 15;198(2):301-7. doi: 10.1042/bj1980301.
The glucocorticoid receptor activity that can be detected in the liver from 15-day foetal rats would appear to be associated with the haemopoietic cells. In hepatocytes, purified by culture for 1-2 days from 15-day foetal rats, the glucocorticoid receptor activity is low and dexamethasone does not induce the enzyme tyrosine aminotransferase. If culture is continued both receptor activity and steroid responsiveness are acquired. Cultured hepatocytes from 19-day foetal liver contain receptor from the first day of culture and, furthermore, the subsequent level of response to glucocorticoids is directly correlated with the actual receptor concentration. It would appear that the glucocorticoid receptor is not acquired by hepatocytes until after 18 days of gestation. Nevertheless, the fact that bromodeoxyuridine has no effect on the rate of accumulation of receptor in hepatocytes suggests that the differentiative event leading to the subsequent appearance of the receptor has already occurred before day 15 of gestation. However, the acquisition of the receptor would appear to be dependent on mitosis as cytosine arabinoside can inhibit the process.
在15日龄胎鼠肝脏中可检测到的糖皮质激素受体活性似乎与造血细胞有关。从15日龄胎鼠中培养1 - 2天纯化得到的肝细胞，其糖皮质激素受体活性较低，地塞米松也不会诱导酪氨酸转氨酶。如果继续培养，受体活性和类固醇反应性都会获得。来自19日龄胎肝的培养肝细胞从培养第一天起就含有受体，此外，随后对糖皮质激素的反应水平与实际受体浓度直接相关。糖皮质激素受体似乎在妊娠18天后才被肝细胞获得。然而，溴脱氧尿苷对肝细胞中受体积累速率没有影响这一事实表明，导致随后受体出现的分化事件在妊娠第15天之前就已经发生。然而，受体的获得似乎依赖于有丝分裂，因为阿糖胞苷可以抑制这一过程。
