多位点序列分型方案的开发。
Development of a multilocus sequence typing scheme.
作者信息
Arcari Gabriele, De Francesco Alice, Polani Riccardo, Carattoli Alessandra, Capitani Valerio
机构信息
Department of Medicine and Technological Innovation, University of Insubria, Varese, Italy.
Ospedale di Circolo e Fondazione Macchi, Laboratory of Medical Microbiology and Virology, Varese, Italy.
出版信息
Front Microbiol. 2024 Oct 31;15:1493621. doi: 10.3389/fmicb.2024.1493621. eCollection 2024.
INTRODUCTION
The genus is assuming greater clinical relevance among infections caused by also because of its intrinsic and acquired resistance to last-resort antibiotics. However, despite having been known and studied for over 50 years, genomics and taxonomy of the genus are currently undergoing a deep rearrangement. In this study we aim to outline and characterized the species.
METHODS
We retrieved from the GenBank database all genomes labelled as and performed a comprehensive genome-based species definition founded on average nucleotide identity (ANI) and on alignment-free approaches.
RESULTS
After defining the genomes assuredly identifiable as , we devised a MultiLocus Sequence Typing (MLST) and a core-genome MLST (cgMLST) schemes, based on 7 and 2,296 loci respectively.
DISCUSSION
This work hence provides a framework for understanding the role of and of other members of this genus, which should be considered as emerging multidrug-resistant pathogens.
引言
该属在由其引起的感染中具有越来越大的临床相关性，这也归因于其对最后手段抗生素的固有和获得性耐药性。然而，尽管该属已被认识和研究了50多年，但其基因组学和分类学目前正在经历深刻的重新排列。在本研究中，我们旨在概述并表征该属物种。
方法
我们从GenBank数据库中检索了所有标记为该属的基因组，并基于平均核苷酸同一性（ANI）和无比对方法进行了全面的基于基因组的物种定义。
结果
在确定了肯定可鉴定为该属的基因组后，我们分别基于7个和2296个基因座设计了多位点序列分型（MLST）和核心基因组MLST（cgMLST）方案。
讨论
因此，这项工作为理解该属及其其他成员的作用提供了一个框架，这些成员应被视为新兴的多重耐药病原体。
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