Division of Materials Science and Chemical Engineering, Yokohama National University, Yokohama, Kanagawa, Japan.
Department of Physiology, Anatomy, and Microbiology, La Trobe University, Bundoora, VIC, Australia.
BMC Genom Data. 2023 Sep 1;24(1):49. doi: 10.1186/s12863-023-01153-2.
Providencia is a genus of gram-negative bacteria within the order Enterobacterales, closely related to Proteus and Morganella. While ubiquitous in the environment, some species of Providencia, such as P. rettgeri and P. stuartii, are considered emerging nosocomial pathogens and have been implicated in urinary tract infection, gastrointestinal illness, and travelers' diarrhea. Given their intrinsic resistance to many commonly used antibiotics, this study aimed to isolate and sequence bacteriophages targeting a clinical P. rettgeri isolate.
Here we report the complete genome sequence of three novel Providencia phages, PibeRecoleta, Stilesk and PatoteraRojo, which were isolated against a clinical P. rettgeri strain sourced from a patient in a metropolitan hospital in Victoria, Australia. The three phages contain dsDNA genomes between 60.7 and 60.9 kb in size and are predicted to encode between 72 and 73 proteins. These three new phages, which share high genomic similarity to two other Providencia phages previously isolated on P. stuartii, serve as important resources in our understanding about Providencia bacteriophages and the potential for future phage-based biotherapies.
普罗威登斯菌是肠杆菌目革兰氏阴性菌属中的一种，与普通变形杆菌和摩根菌属密切相关。虽然在环境中普遍存在，但某些普罗威登斯菌物种，如雷特氏普罗威登斯菌和斯图尔特氏普罗威登斯菌，被认为是新兴的医院获得性病原体，并与尿路感染、胃肠道疾病和旅行者腹泻有关。鉴于它们对许多常用抗生素具有固有抗性，本研究旨在分离和测序针对临床雷特氏普罗威登斯菌分离株的噬菌体。
在这里，我们报告了三个新的普罗威登斯噬菌体 PibeRecoleta、Stilesk 和 PatoteraRojo 的完整基因组序列，这些噬菌体是针对从澳大利亚维多利亚州一家大都市医院的患者中分离出的临床雷特氏普罗威登斯菌菌株而分离出来的。这三个噬菌体的 dsDNA 基因组大小在 60.7 到 60.9 kb 之间，预计编码 72 到 73 种蛋白质。这三个新的噬菌体与之前在斯图尔特氏普罗威登斯菌上分离得到的两个其他普罗威登斯噬菌体具有高度的基因组相似性，它们是我们了解普罗威登斯噬菌体的重要资源，也为未来基于噬菌体的生物疗法提供了潜力。
