Opt Lett. 2024 Nov 15;49(22):6449-6452. doi: 10.1364/OL.538165.
This study introduces an ultra-wide field (UWF) and high-resolution swept-source optical coherence tomographic angiography (OCTA) system for rat retinal imaging. Using an asymmetrical optics design, the system achieves unprecedented details of retinal structures and vascular plexuses over a large field of view (112°) in a single-shot acquisition. Views of single-nerve fiber bundles and single capillary vessels are consistently visible over a 112° field of view. The system has a long imaging range and high penetration and allows a full view of vitreous hyaloid vessels, retina, choroid, sclera, and posterior ciliary arteries, down to sub-sclera connective tissues. In a longitudinal study of oxygen-induced retinopathy (OIR) in rats, the system successfully revealed the progression and regression of OIR-related vascular pathologies, such as ischemia, neovascularization, and tortuosity. To our knowledge, this novel UWF-OCT/OCTA prototype designed for rat retinal imaging will be a vital tool for monitoring disease progression and evaluating therapeutic interventions in preclinical models.
本研究介绍了一种用于大鼠视网膜成像的超广角（UWF）和高分辨率扫频源光相干断层扫描血管造影（OCTA）系统。该系统采用非对称光学设计，在单次采集时实现了前所未有的视网膜结构和血管丛细节，视野达到 112°。在 112°的视野中，始终可以看到单个神经纤维束和单个毛细血管的图像。该系统具有长的成像范围和高的穿透深度，可以全面观察玻璃体血管、视网膜、脉络膜、巩膜和睫后动脉，直至巩膜下结缔组织。在一项针对氧诱导视网膜病变（OIR）的大鼠纵向研究中，该系统成功揭示了 OIR 相关血管病变（如缺血、新生血管形成和迂曲）的进展和消退。据我们所知，这种专为大鼠视网膜成像设计的新型 UWF-OCT/OCTA 原型将成为监测疾病进展和评估临床前模型中治疗干预的重要工具。
