Canela E I, Franco R
Biochem J. 1986 Jan 15;233(2):599-605. doi: 10.1042/bj2330599.
A method is described for fitting the velocities obtained from progress curves to a steady-state rate equation. It is based on the method of Markus & Plesser [(1981) in Kinetic Data Analysis: Design and Analysis of Enzyme and Kinetic Data (Edrenyi, ed.), pp. 317-339, Plenum Press, New York]. The obstacle of needing good initial estimates of kinetic parameters is removed by using the parameters provided graphically by a minor modification of the method of Yun & Suelter [(1977) Biochim, Biophys. Acta 480, 1-13]. This progress-curved-based method allows the same discrimination among rival models as do the initial-velocity-based methods, with a great saving of experimental time. The BASIC and FORTRAN 77 programs are deposited as Supplementary Publication SUP 50132 (17 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1986) 233, 5-6.
本文描述了一种将从进程曲线获得的速度拟合到稳态速率方程的方法。它基于Markus和Plesser的方法[(1981年载于《动力学数据分析：酶和动力学数据的设计与分析》(埃德里尼编)，第317 - 339页，普伦纽姆出版社，纽约)]。通过使用对Yun和Suelter方法[(1977年，《生物化学与生物物理学报》480卷，第1 - 13页)]稍加修改后以图形方式提供的参数，消除了对动力学参数进行良好初始估计的障碍。这种基于进程曲线的方法与基于初速度的方法一样，能够对竞争模型进行相同的区分，同时大大节省了实验时间。BASIC和FORTRAN 77程序作为补充出版物SUP 50132(17页)存放在英国西约克郡韦瑟比市波士顿温泉镇英国国家图书馆(出借部)，邮编LS23 7BQ，可按《生物化学杂志》(1986年)233卷第5 - 6页所示条件从该处获取副本。
