Laboratory of Infections, Carcinogenesis and Regeneration, Medical Biotechnology Section, Department of Biological and Chemical Engineering, Aarhus University, Aarhus, Denmark.
Department of Microbiology, University of Würzburg, Würzburg, Germany.
Sci Data. 2024 Nov 16;11(1):1238. doi: 10.1038/s41597-024-04081-7.
Gastroesophageal disorders and cancers impose a significant global burden. Particularly, the prevalence of esophageal adenocarcinoma (EAC) has increased dramatically in recent years. Barrett's esophagus, a precursor of EAC, features a unique tissue adaptation at the gastroesophageal squamo-columnar junction (GE-SCJ), where the esophagus meets the stomach. Investigating the evolution of GE-SCJ and understanding dysregulation in its homeostasis are crucial for elucidating cancer pathogenesis. Here, we present the technical quality of the comprehensive single-cell RNA sequencing (scRNA-seq) dataset from mice that captures the transcriptional dynamics during the development of the esophagus, stomach and the GE-SCJ at embryonic, neonatal and adult stages. Through integration with external scRNA-seq datasets and validations using organoid and animal models, we demonstrate the dataset's consistency in identified cell types and transcriptional profiles. This dataset will be a valuable resource for studying developmental patterns and associated signaling networks in the tissue microenvironment. By offering insights into cellular programs during homeostasis, it facilitates the identification of changes leading to conditions like metaplasia and cancer, crucial for developing effective intervention strategies.
胃食管疾病和癌症在全球造成了巨大负担。特别是食管腺癌 (EAC) 的患病率近年来急剧上升。巴雷特食管是 EAC 的前身，其特征是在胃食管鳞柱状交界处 (GE-SCJ) 出现独特的组织适应，食管与胃在此处相遇。研究 GE-SCJ 的演变并了解其体内平衡的失调对于阐明癌症发病机制至关重要。在这里，我们展示了来自小鼠的综合单细胞 RNA 测序 (scRNA-seq) 数据集的技术质量，该数据集在胚胎、新生儿和成年阶段捕获了食管、胃和 GE-SCJ 发育过程中的转录动态。通过与外部 scRNA-seq 数据集的整合以及使用类器官和动物模型进行验证，我们证明了数据集在鉴定的细胞类型和转录谱方面的一致性。该数据集将成为研究组织微环境中发育模式和相关信号网络的宝贵资源。通过提供对体内平衡期间细胞程序的深入了解，它有助于识别导致化生和癌症等情况的变化，这对于开发有效的干预策略至关重要。
