Zhou Gongting, Lin Xiang, Li Hongzheng, Sun Weijian, Li Wenzhao, Zhang Qingfei, Bian Feika, Lin Ji
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027, China.
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325001, China.
Biosens Bioelectron. 2025 Feb 1;269:116944. doi: 10.1016/j.bios.2024.116944. Epub 2024 Nov 14.
Chemotherapy is one of the main therapeutic methods for tumor treatment. However, improving the accuracy of personalized medication for chemotherapy remains challenging. In this study, we developed a novel microfluidic chip that features herringbone protrusions and three-dimensional (3D) microcolumn holes created from microcolumn arrays. This design allows for precise control over the size and number of 3D tumor cell spheroids. As tumor cells aggregate into clusters within the chip, an integrated microfluidic mixer enhances liquid mixing and improves contact between the spheroids and the culture medium, promoting their growth. By combining this 3D spheroid approach with a concentration gradient mixer, we effectively conducted dynamic and high-throughput evaluations of anti-tumor drugs. The chip successfully identified varying sensitivities of tumor cells from different patients to these drugs, aligning with clinical observations from postoperative follow-ups. These features indicated that the tumor cell spheroid integrated microfluidic chip is effective for drug evaluation methodologies and holds promising implications for clinical applications.
化疗是肿瘤治疗的主要方法之一。然而，提高化疗个性化用药的准确性仍然具有挑战性。在本研究中，我们开发了一种新型微流控芯片，其具有人字形突起以及由微柱阵列形成的三维（3D）微柱孔。这种设计能够精确控制3D肿瘤细胞球体的大小和数量。当肿瘤细胞在芯片内聚集成团时，集成的微流控混合器可增强液体混合，并改善球体与培养基之间的接触，促进其生长。通过将这种3D球体方法与浓度梯度混合器相结合，我们有效地进行了抗肿瘤药物的动态和高通量评估。该芯片成功识别了不同患者的肿瘤细胞对这些药物的不同敏感性，与术后随访的临床观察结果一致。这些特性表明，肿瘤细胞球体集成微流控芯片对于药物评估方法是有效的，并且对临床应用具有广阔的前景。
