da Silva Prudêncio Rafael, Sousa Antonio Kleiton de, Silva Denise Mayara Melo, Santos Ferreira Jayro Dos, Rocha Danyela Maria Leal, Almeida Viviane Pinheiro Alves de, Silva Sousa João Janilson da, Sousa Stefany Guimarães, da Silva Tino Marcos Lino, Dos Santos Carvalho André, Cruz Júnior José Simião da, Alves Even Herlany Pereira, Vasconcelos Daniel Fernando Pereira, Bezerra Roosevelt D S, França Dourado Flaviane de, Oliveira Aline Lima de, Cabral Wanessa Feliz, Souza de Almeida Leite José Roberto de, da Silva Durcilene Alves, Nascimento Junior Elias Borges do, Brito Tarcísio Vieira de, Aguiar Magalhães Diva de, Barbosa André Luiz Dos Reis
Laboratory of Experimental Physiopharmacology (LAFFEX), Parnaiba Delta Federal University (UFDPar), Parnaiba, Piaui, Brazil.
Laboratory of Histological Analysis and Preparation (LAPHis), Parnaiba Delta Federal University (UFDPar), Parnaiba, Piaui, Brazil.
Carbohydr Res. 2025 Jan;547:109322. doi: 10.1016/j.carres.2024.109322. Epub 2024 Nov 15.
Seaweeds are natural sources of sulfated polysaccharides (SPs), biopolymers with remarkable pharmacological properties, including biological actions capable of attenuating components of the inflammatory process such as edema, cytokines, cell migration and pain. Our results confirm that SPs obtained from Gracilaria domingensis (SP-GD) are agarans, primarily composed of residues of β-d-galactopyranose 6-sulfate and 3,6-anhydro-α-l-galactopyranose. Specifically, SP-GD at a dose of 10 mg/kg was effective in significantly reducing paw edema induced by carrageenan or histamine, serotonin, bradykinin, 48/80 and prostaglandin E2. SP-GD (10 mg/kg) was also able to reduce neutrophil migration and the activity of the myeloperoxidase enzyme in carrageenan-induced peritonitis, as well as conserve glutathione concentration and reduce malondialdehyde levels in the animals' peritoneal fluid. Furthermore, it showed antinociceptive action in the abdominal writhing test induced by acetic acid and in the paw licking test induced by formalin. Thus, the results obtained allow us to infer that SPs extracted from G. domingensis at a dose of 10 mg/kg have anti-inflammatory effects by reducing neutrophil migration and modulating the activity of vasoactive mediators and antinociceptive effects by acting, at least in part, through a peripheral mechanism dependent on the negative modulation of inflammatory mediators.
海藻是硫酸化多糖（SPs）的天然来源，硫酸化多糖是具有显著药理特性的生物聚合物，其生物活性包括能够减轻炎症过程中的多种成分，如水肿、细胞因子、细胞迁移和疼痛。我们的研究结果证实，从多米尼克江蓠（Gracilaria domingensis）中提取的SPs（SP-GD）是琼胶聚糖，主要由β-d-吡喃半乳糖6-硫酸酯和3,6-脱水-α-l-吡喃半乳糖的残基组成。具体而言，剂量为10mg/kg的SP-GD能有效显著减轻角叉菜胶或组胺、5-羟色胺、缓激肽、48/80和前列腺素E2诱导的爪部水肿。SP-GD（10mg/kg）还能够减少角叉菜胶诱导的腹膜炎中中性粒细胞的迁移以及髓过氧化物酶的活性，同时能维持动物腹膜液中谷胱甘肽的浓度并降低丙二醛水平。此外，在乙酸诱导的腹部扭体试验和福尔马林诱导的舔爪试验中，它显示出抗伤害感受作用。因此，所获得的结果使我们能够推断，从多米尼克江蓠中提取的剂量为10mg/kg的SPs通过减少中性粒细胞迁移和调节血管活性介质的活性而具有抗炎作用，并且通过至少部分地依赖于对炎症介质的负调节的外周机制发挥抗伤害感受作用。
