College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, 710119, China; Engineering Research Center of High-Valued Utilization of Fruit Resources in Western China, Ministry of Education, Xi'an, 710119, China.
College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China.
Plant Physiol Biochem. 2024 Dec;217:109285. doi: 10.1016/j.plaphy.2024.109285. Epub 2024 Nov 9.
The 'Xuxiang' kiwifruit, a leading cultivar in China known for its high quality and yield, experiences quality degradation due to vibration stress during postharvest transportation. This study simulated the postharvest transportation vibrations of 'Xuxiang' kiwifruits to investigate the effects on the fruit quality and physiology. Different vibration intensities (0.26, 0.79, and 1.5 m s) and durations (0, 24, 48, 72, and 96 h) were applied to analyze the quality, physiological and transcriptomic changes of fruits after vibration stress, as well as the association between quality deterioration, gene networks, and key genes. Results indicated that vibration stress significantly accelerated the deterioration of fruit quality and induced physiological changes. As vibration intensity and duration increased, there was a rapid decrease in fruit firmness and an increase in weight loss, soluble solid content, relative conductivity, ethylene production, respiratory rate, and malondialdehyde levels. The most severe deterioration in fruit quality occurred at a vibration intensity of 1.5 m s. Transcriptome sequencing analysis was conducted on samples from different durations of exposure to the 1.5 m s vibration intensity. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses identified key genes associated with ethylene metabolism and softening. Weighted Gene Co-Expression Network Analysis (WGCNA) and correlation analysis further determined that 24 of these genes were regulated by vibrational stress, impacting ethylene metabolism and cell wall degradation. Vibration stress induced changes in genes related to ethylene metabolism and cell wall degradation, promoting lipid peroxidation and respiratory changes, which compromise cell membrane integrity and lead to quality deterioration. Compared with untreated fruits, vibration stress caused the quality deterioration, physiological changes and transcriptional regulation of kiwifruits, indicating that kiwifruits respond to vibration stress through multiple aspects. It proposes a fresh outlook on the understanding of the mechanism of transport vibration stress and further illustrates the importance of monitoring vibration intensity and duration as well as reducing vibration.
“徐香”猕猴桃是中国的主栽品种之一，以其高品质和高产量而闻名，但在采后运输过程中会因振动而导致品质下降。本研究模拟了“徐香”猕猴桃的采后运输振动，以研究其对果实品质和生理的影响。不同的振动强度（0.26、0.79 和 1.5 m s）和持续时间（0、24、48、72 和 96 h）应用于分析振动胁迫后果实的品质、生理和转录组变化，以及品质劣变、基因网络和关键基因之间的关联。结果表明，振动胁迫显著加速了果实品质的劣变，并诱导了生理变化。随着振动强度和时间的增加，果实硬度迅速下降，失重率、可溶性固形物含量、相对电导率、乙烯生成量、呼吸速率和丙二醛水平增加。在振动强度为 1.5 m s 时，果实品质劣变最为严重。对暴露于 1.5 m s 振动强度不同时间的样品进行转录组测序分析。京都基因与基因组百科全书（KEGG）和基因本体论（GO）富集分析确定了与乙烯代谢和软化相关的关键基因。加权基因共表达网络分析（WGCNA）和相关性分析进一步确定，其中 24 个基因受振动胁迫调控，影响乙烯代谢和细胞壁降解。振动胁迫诱导与乙烯代谢和细胞壁降解相关的基因发生变化，促进脂质过氧化和呼吸变化，破坏细胞膜完整性，导致品质劣变。与未处理的果实相比，振动胁迫导致猕猴桃的品质劣变、生理变化和转录调控，表明猕猴桃通过多个方面对振动胁迫做出响应。这为理解运输振动胁迫机制提供了新的视角，并进一步说明了监测振动强度和时间以及减少振动的重要性。
