Chen Pei, Sang Ee, Chen Huanhuan, Meng Qi, Liu Huiping
School of Pharmacy & School of Biological and Food Engineering, Changzhou University, Changzhou 213164, PR China..
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science &Technology, Tianjin 300457, PR China.
Int J Biol Macromol. 2024 Dec;283(Pt 3):137726. doi: 10.1016/j.ijbiomac.2024.137726. Epub 2024 Nov 15.
Dandelion polysaccharides contribute to a variety of biological activities. This study evaluated the effect of different extraction temperatures (4 °C and 80 °C) on the structural characteristics and antioxidant activity of dandelion leaf polysaccharides (DLP). The findings demonstrated that the extraction efficiency improved at the higher temperature, while molecular weight exist a trend of degradation with increasing extraction temperature. Ion chromatography (IC) analysis indicated that the polysaccharides DLP4 and DLP80 were structurally complex heteropolysaccharides mainly composed of galactose, arabinose, glucose and mannose, with galactose and arabinose dominating. FT-IR and methylation analysis revealed that DLP4 and DLP80 had similar chemical structures and branches. DLP4 contained a higher amount of 6-Galactose. Microstructure analysis showed that heat treatment caused conformational changes in DLP4 and DLP80. Both had excellent free radical scavenging ability including DPPH·, ABTS, OH· and reducing power. The Reactive Oxygen Species assay indicated that the protective effect of DLP4 against HO-induced oxidative damage in vitro was stronger than that of DLP80. Superoxide dismutase (SOD) and malondialdehyde (MDA) measurements also confirmed that the antioxidant effect of DLP4 was more prominent. Overall, low temperature extracted DLP can be used as an antioxidant in the areas of food, medicine and biomaterials.
蒲公英多糖具有多种生物活性。本研究评估了不同提取温度（4℃和80℃）对蒲公英叶多糖（DLP）结构特征和抗氧化活性的影响。研究结果表明，较高温度下提取效率提高，而分子量随提取温度升高存在降解趋势。离子色谱（IC）分析表明，多糖DLP4和DLP80是结构复杂的杂多糖，主要由半乳糖、阿拉伯糖、葡萄糖和甘露糖组成，其中半乳糖和阿拉伯糖占主导。傅里叶变换红外光谱（FT-IR）和甲基化分析表明，DLP4和DLP80具有相似的化学结构和分支。DLP4含有较高含量的6-半乳糖。微观结构分析表明，热处理导致DLP4和DLP80的构象发生变化。二者均具有优异的自由基清除能力，包括对DPPH·、ABTS、OH·的清除能力以及还原力。活性氧（ROS）检测表明，DLP4对体外HO诱导的氧化损伤的保护作用强于DLP80。超氧化物歧化酶（SOD）和丙二醛（MDA）测定也证实DLP4的抗氧化作用更显著。总体而言，低温提取的DLP可作为食品、医药和生物材料领域的抗氧化剂。
