Ni Shuang, Zhao Haolan, Yang Shaohua, Cui Kai
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Ultrason Sonochem. 2025 May;116:107329. doi: 10.1016/j.ultsonch.2025.107329. Epub 2025 Mar 28.
In this study, ultrasonic-assisted enzymatic extraction (UAEE) method was used to obtain the small molecule dandelion polysaccharides. Based on single-factor experiments, the process of extraction the extraction conditions were further optimized by response surface method (RSM) combined with the Box-Behnken design (BBD). The results showed that in combination with enzymes, with a ratio of 1:32 (g/ml), an enzymolysis temperature 55 °C, an ultrasonic temperature 75℃ and the ultrasonic time 55 min. The maximum extraction yield of crude polysaccharides was 3.127 %.After purification by DEAE-cellulose chromatography and Sephadex G-75 gel filtration, a novel polysaccharide (DANP-Ⅱ) was extracted and the results of high performance liquid chromatography (HPLC) indicated its average molecular weight was 3.373 kDa. Gas chromatography (GC) analysis showed that DANP-Ⅱ was mainly contained by glucose and little of glucuronic acid, mannose, galactose, and arabinose. In vitro antioxidant experiments have demonstrated that DANP-Ⅱ can alleviate H2O2-induced cellular damage, reduce apoptosis rates, and exhibit robust antioxidant activity. Hence, DANP-Ⅱ can be utilized as a natural antioxidant to enhance protection against oxidative stress, providing theoretical guidance for the utilization of low-molecular-weight dandelion polysaccharides.
本研究采用超声辅助酶提取法（UAEE）制备小分子蒲公英多糖。在单因素实验基础上，结合Box-Behnken设计（BBD），采用响应面法（RSM）进一步优化提取条件。结果表明，酶用量为1:32（g/ml），酶解温度55℃，超声温度75℃，超声时间55min时，粗多糖的最大提取率为3.127%。经DEAE-纤维素柱层析和Sephadex G-75凝胶过滤纯化后，得到一种新型多糖（DANP-Ⅱ），高效液相色谱（HPLC）结果表明其平均分子量为3.373 kDa。气相色谱（GC）分析表明，DANP-Ⅱ主要由葡萄糖组成，含有少量葡萄糖醛酸、甘露糖、半乳糖和阿拉伯糖。体外抗氧化实验表明，DANP-Ⅱ可减轻H2O2诱导的细胞损伤，降低凋亡率，具有较强的抗氧化活性。因此，DANP-Ⅱ可作为天然抗氧化剂增强对氧化应激的保护作用，为低分子量蒲公英多糖的利用提供理论指导。
