Murugesan Srimathi, Balasubramanian Satheeswaran, Perumal Ekambaram
Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, India.
Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, India.
Chem Biol Interact. 2025 Jan 5;405:111311. doi: 10.1016/j.cbi.2024.111311. Epub 2024 Nov 15.
Copper oxide nanoparticles (CuO NPs) are widely employed in various industrial and biomedical applications owing to their enhanced physicochemical characteristics. However, concerns regarding their adverse effects on biological systems upon entering the environment remain unexplored. The generation of reactive oxygen species (ROS) is one of the primary mechanisms in CuO NPs induced toxicity. This meta-analysis was conducted to assess the associative link between CuO NPs exposure and ROS generation. A literature survey was performed in PubMed, Web of Science, Scopus, and Google Scholar, following PRISMA guidelines. After comprehensive initial and primary screening, 28 in vitro studies were selected for meta-analysis. Overall, our results show a substantial increase of ROS in the experimental group when compared to control (SMD = 3.3; 95 % CI: 2.82-3.77, p = 0.00001), with substantial heterogeneity (82 %). Subgroup analysis revealed that larger-sized NPs, higher dosages, and longer exposure duration were associated with ROS generation. Meta-regression analysis identified size, and dosage as significant factors influencing ROS levels. Sensitivity analysis revealed an outlier study and the funnel plot results suggested potential publication bias. Overall, our results provide valuable insights of CuO NPs induced ROS generation, and the relation of variables such as size, dose, and duration in nanotoxicity assessments.
氧化铜纳米颗粒（CuO NPs）因其增强的物理化学特性而广泛应用于各种工业和生物医学领域。然而，关于其进入环境后对生物系统的不利影响仍未得到充分研究。活性氧（ROS）的产生是CuO NPs诱导毒性的主要机制之一。本荟萃分析旨在评估CuO NPs暴露与ROS产生之间的关联。按照PRISMA指南，在PubMed、科学网、Scopus和谷歌学术上进行了文献检索。经过全面的初步筛选和主要筛选，选择了28项体外研究进行荟萃分析。总体而言，我们的结果表明，与对照组相比，实验组中的ROS显著增加（标准化均数差=3.3；95%置信区间：2.82 - 3.77，p = 0.00001），且存在显著异质性（82%）。亚组分析显示，较大尺寸的纳米颗粒、较高剂量和较长暴露时间与ROS产生有关。荟萃回归分析确定尺寸和剂量是影响ROS水平的重要因素。敏感性分析发现一项异常值研究，漏斗图结果表明可能存在发表偏倚。总体而言，我们的结果为CuO NPs诱导的ROS产生以及纳米毒性评估中尺寸、剂量和持续时间等变量之间的关系提供了有价值的见解。
