Department of Biotechnology, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia.
Ethiopian Institute of Agricultural Research (EIAR), Addis Ababa, Ethiopia.
World J Microbiol Biotechnol. 2024 Nov 18;40(12):384. doi: 10.1007/s11274-024-04189-3.
Understanding the microbial ecology of landfills is crucial for improving waste management strategies and utilizing the potential of these microbial communities for biotechnological applications. This study aimed to conduct a comprehensive taxonomic and functional profiling of the microbial community present in the Addis Ababa municipal solid waste dumpsite using a shotgun metagenomics sequencing approach. The taxonomic analysis of the sample revealed the significant presence of bacteria, with the Actinomycetota (56%), Pseudomonadota (23%), Bacillota (3%), and Chloroflexota (3%) phyla being particularly abundant. The most abundant KEGG categories were carbohydrates metabolism, membrane transport, signal transduction, and amino acid metabolism. The biodegradation and metabolism of xenobiotics, as well as terpenoids and polyketides, were also prevalent. Moreover, the Comprehensive Antibiotic Resistance Database (CARD) identified 52 antibiotic resistance gene (ARG) subtypes belonging to 14 different drug classes, with the highest abundances observed for glycopeptide, phosphonic acid, and multidrug resistance genes. Actinomycetota was the dominant phylum harboring ARGs, followed by Pseudomonadota and Chloroflexota. This study offers valuable insights into the taxonomic and functional diversity of the microbial community in the Addis Ababa municipal solid waste dumpsite. It sheds light on the widespread presence of metabolically versatile microbes, antibiotic resistance genes, mobile genetic elements, and pathogenic bacteria. This understanding can contribute to the creation of efficient waste management strategies and the investigation of possible biotechnological uses for these microbial communities.
了解垃圾填埋场的微生物生态学对于改进废物管理策略以及利用这些微生物群落的潜力进行生物技术应用至关重要。本研究旨在采用高通量宏基因组测序方法，对亚的斯亚贝巴市固体废物倾倒场中存在的微生物群落进行全面的分类和功能分析。样品的分类分析显示，细菌的存在非常显著，其中放线菌门（56%）、假单胞菌门（23%）、芽孢杆菌门（3%）和绿弯菌门（3%）特别丰富。最丰富的 KEGG 类别为碳水化合物代谢、膜转运、信号转导和氨基酸代谢。此外，还存在对异生物质、萜类化合物和聚酮化合物的生物降解和代谢。此外，综合抗生素耐药性数据库（CARD）鉴定出属于 14 种不同药物类别的 52 种抗生素耐药基因（ARG）亚型，其中糖肽、膦酸和多药耐药基因的丰度最高。放线菌门是携带 ARG 的主要门，其次是假单胞菌门和绿弯菌门。本研究提供了对亚的斯亚贝巴市固体废物倾倒场微生物群落的分类和功能多样性的有价值的见解。它揭示了代谢多功能微生物、抗生素耐药基因、移动遗传元件和病原菌的广泛存在。这种理解可以为制定有效的废物管理策略以及研究这些微生物群落的可能生物技术应用做出贡献。
