Azhigirova M A, Viazova E P, Khachatur'ian A A
Biull Eksp Biol Med. 1986 Mar;101(3):300-2.
Several ketocarbonic acids covalently attached to the protein moiety of hemoglobin were tested as reversible oxygenation regulators. Two monocarbonic acids (glycoxylic and piruvic) and bicarbonic--alpha-ketoglutaric-acid were used for remoglobin modification. Functional properties and isoelectrical characteristics of modified hemoglobin were recorded. The binding of these acids to hemoglobin was shown to affect the net charge of hemoglobin molecule and the nature of its interaction with allosteric effectors (O2; CI-; H+). Under the influence of glyoxylic acid, the gas-transporting characteristics of hemoglobin underwent most distinct changes approaching those of the human whole blood.
对几种与血红蛋白蛋白质部分共价连接的酮碳酸作为可逆氧合调节剂进行了测试。使用两种一碳酸（乙醛酸和丙酮酸）以及碳酸氢-α-酮戊二酸对血红蛋白进行修饰。记录了修饰后血红蛋白的功能特性和等电特性。结果表明，这些酸与血红蛋白的结合会影响血红蛋白分子的净电荷及其与变构效应剂（O2；Cl-；H+）相互作用的性质。在乙醛酸的影响下，血红蛋白的气体运输特性发生了最明显的变化，接近人类全血的特性。
