Kalaki Niloufar Sadat, Ahmadzadeh Mozhgan, Dehghan Mandana, Shahabi Rabori Venus, Davoudi Sima, Afkhami Hamed
Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Biochem Biophys Rep. 2024 Nov 1;40:101863. doi: 10.1016/j.bbrep.2024.101863. eCollection 2024 Dec.
Breast cancer (BC) presents as a worldwide challenge, known as the most frequently diagnosed cancer in women. In 2022, BC was diagnosed in 2.3 million women with 670,000 deaths globally. In this research, our objective was to examine the CST7 and has-miR-4793-5p gene expression in BC tumor tissues and adjacent normal tissues. Using GSE57897 gene expression data from 422 BC samples and 31 breast samples from healthy controls which was based on the Platform GPL18722 (spotted oligonucleotide Homo sapiens microRNA (miRNA) array) in the Gene Expression Omnibus (GEO) and compare with miRNAs with a conserved target location on CST7 mRNA were found using databases. The study population included 60 fresh BC tissue samples and adjacent normal tissues as control. The Quantitative Real-Time PCR was used to evaluate the expression levels of CST7 and has-miR-4793-5p in the breast tissues. The present study, found that CST7 and hsa-miR-4793-5p were significantly increased in tumoral tissues in compare to normal tissues. Further analysis revealed a remarkable association between CST7 and hsa-miR-4793-5p gene expression alteration. ROC curve analysis demonstrated high accuracy for CST7 expression in BC tumors. Comparison of gene expression between different stages and patient family history showed significant findings. Due to the high sensitivity and specificity of the expression changes of these two genes, they are suitable candidates for further investigations to be considered as part of a diagnosis and prognosis panel.
乳腺癌是一项全球性挑战，是女性中最常被诊断出的癌症。2022年，全球有230万女性被诊断出患有乳腺癌，其中67万人死亡。在本研究中，我们的目的是检测CST7和has-miR-4793-5p基因在乳腺癌肿瘤组织及相邻正常组织中的表达情况。使用来自基因表达综合数据库（GEO）中基于GPL18722平台（点样寡核苷酸人类miRNA阵列）的422例乳腺癌样本和31例健康对照乳腺样本的GSE57897基因表达数据，并通过数据库查找与CST7 mRNA具有保守靶位点的miRNA进行比较。研究人群包括60例新鲜乳腺癌组织样本及作为对照的相邻正常组织。采用定量实时PCR评估乳腺组织中CST7和has-miR-4793-5p的表达水平。本研究发现，与正常组织相比，CST7和hsa-miR-4793-5p在肿瘤组织中显著升高。进一步分析显示CST7和hsa-miR-4793-5p基因表达改变之间存在显著关联。ROC曲线分析表明CST7在乳腺癌肿瘤中的表达具有较高准确性。不同分期和患者家族史之间的基因表达比较显示出显著结果。由于这两个基因表达变化具有高敏感性和特异性，它们是进一步研究的合适候选基因，可考虑作为诊断和预后指标的一部分。
