Chen Hsuan-Pu, Chan Fang-Tse, Shiao Shiuh-Feng, Chiu Ming-Chung
Department of Entomology, National Taiwan University, Taipei, Taiwan Department of Entomology, National Taiwan University Taipei Taiwan.
Wildlife Rescue and Research Center, Taiwan Biodiversity Research Institute, Nantou, Taiwan Wildlife Rescue and Research Center, Taiwan Biodiversity Research Institute Nantou Taiwan.
Biodivers Data J. 2024 Nov 8;12:e137532. doi: 10.3897/BDJ.12.e137532. eCollection 2024.
The genus Nitzsch, 1818 comprises small ectoparasites that feed on the blood of juvenile avians. They are characterised by dealated adults with setose abdominal intersegmental membranes. Maa, 1968 was previously recorded in Malaysia and the Ryukyu Islands of Japan, parasitising two owl species: (Horsfield, 1821) and (Cassin, 1852). This study confirms the occurrence of in Taiwan and presents a new host record, along with barcode sequences. Additionally, the study also elucidates the difficulties posed by blood meal contamination and pseudogene amplification as confounding factors intrinsic to the molecular taxonomic delineation of via universal DNA barcoding primers.
The following new information regarding is provided in this study: is first recorded in Taiwan, filling the gap in its East Asian distribution. This is also the first record of Carnidae from Taiwan. (Hodgson, 1836) (Aves, Strigidae) is reported as a new host for , identified on a fallen fledgling.Co-amplification of the host's is reported in this study using the universal PCR primer set LCO1490/HCO2198. Additionally, the amplification of a -like pseudogene using a newly-designed primer set is detected through abnormal translated amino acid sequences and the occurrence of a stop codon.New specific primers for the gene of were designed in this study. The new distribution and ecological data of enhance our understanding of this species. The provision of new primers is anticipated to contribute to future studies employing DNA barcoding in bird-parasitic flies.
1818年的尼茨氏属包含以幼年鸟类血液为食的小型体外寄生虫。它们的特征是成虫无翅，腹部节间膜有刚毛。1968年马氏在马来西亚和日本琉球群岛有过记录，寄生于两种猫头鹰：（霍斯菲尔德，1821年）和（卡辛，1852年）。本研究证实了该物种在台湾的出现，并给出了新的宿主记录以及条形码序列。此外，该研究还阐明了血餐污染和假基因扩增作为通过通用DNA条形码引物对该物种进行分子分类鉴定时的内在混杂因素所带来的困难。
本研究提供了以下关于该物种的新信息：该物种首次在台湾被记录，填补了其在东亚分布的空白。这也是台湾首次记录到 Carnidae 科。（ Hodgson，1836年）（鸟纲，草鸮科）被报告为该物种的新宿主，是在一只掉落的雏鸟身上鉴定出来的。本研究报告了使用通用PCR引物组LCO1490/HCO2198对宿主的 进行共扩增。此外，通过异常的翻译氨基酸序列和终止密码子的出现，检测到使用新设计的引物组扩增出了一个类似 的假基因。本研究设计了用于该物种 基因的新特异性引物。该物种新的分布和生态数据增进了我们对该物种的了解。新引物的提供预计将有助于未来在鸟类寄生蝇类中采用DNA条形码技术的研究。
