植入物相关感染的先进诊断技术：超声处理与二硫苏糖醇的协同作用
Advancing Diagnostic Techniques for Implant-Related Infections: the Synergistic Effect of Sonication and Dithiothreitol.
作者信息
Bakalakos Matthaios, Ampadiotaki Margarita-Michaela, Vlachos Christos, Stylianakis Antonios, Sipsas Nikolaos, Pneumaticos Spiros, Vlamis John
机构信息
3rd Orthopaedic Department, National and Kapodistrian University of Athens, KAT General Hospital, Athens, Greece.
2nd Orthopaedic Department, KAT General Hospital, Athens, Greece.
出版信息
Maedica (Bucur). 2024 Sep;19(3):561-565. doi: 10.26574/maedica.2024.19.3.561.
PURPOSE
Implant-related infections represent a significant complication contributing to increased morbidity and mortality. Determining the microbial agent causing the infection is crucial for successful treatment. Despite the incidence of periprosthetic joint infections (PJIs) rising over time, no diagnostic test with 100% sensitivity is available to identify these infections accurately. The present study aims to determine whether combining sonication with Dithiothreitol (DTT) enhances the accuracy and sensitivity in diagnosing implant-related infections.
METHODS
Specifically, the present study included 30 patients, who underwent implant removal due to suspicion of infection. Implants were divided into two segments: one was processed using the sonication method and the other one by combining DTT and sonication.
RESULTS
The mean +/-SD was 81.17 +/- 67.53 CFU/mL for the sonication group and 109.7 +/-62.78 CFU/mL for the combination group.
CONCLUSION
The results of our study indicate that the combination of DTT and sonication increases the colony count by about 28.53 CFU/mL, which enhances the possibility of detecting orthopaedic implant associated infections.
目的
植入物相关感染是导致发病率和死亡率增加的重要并发症。确定引起感染的微生物病原体对于成功治疗至关重要。尽管随着时间的推移，人工关节周围感染（PJI）的发病率有所上升，但尚无100%敏感性的诊断测试可准确识别这些感染。本研究旨在确定超声处理与二硫苏糖醇（DTT）联合使用是否能提高诊断植入物相关感染的准确性和敏感性。
方法
具体而言，本研究纳入了30例因怀疑感染而接受植入物取出术的患者。将植入物分为两段：一段采用超声处理方法，另一段采用DTT与超声联合处理。
结果
超声处理组的平均±标准差为81.17±67.53 CFU/mL，联合处理组为109.7±62.78 CFU/mL。
结论
我们的研究结果表明，DTT与超声联合使用可使菌落计数增加约28.53 CFU/mL，这提高了检测骨科植入物相关感染的可能性。
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