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拟南芥中的大规模表型分析揭示了RNA干扰在多种植物与微生物相互作用中的不同参与情况。

Broad-scale phenotyping in Arabidopsis reveals varied involvement of RNA interference across diverse plant-microbe interactions.

作者信息

Ruf Alessa, Thieron Hannah, Nasfi Sabrine, Lederer Bernhard, Fricke Sebastian, Adeshara Trusha, Postma Johannes, Blumenkamp Patrick, Kwon Seomun, Brinkrolf Karina, Feldbrügge Michael, Goesmann Alexander, Kehr Julia, Steinbrenner Jens, Šečić Ena, Göhre Vera, Weiberg Arne, Kogel Karl-Heinz, Panstruga Ralph, Robatzek Silke

机构信息

LMU Munich Biocenter Planegg Germany.

Unit for Plant Molecular Cell Biology, Institute for Biology I RWTH Aachen University Aachen Germany.

出版信息

Plant Direct. 2024 Nov 15;8(11):e70017. doi: 10.1002/pld3.70017. eCollection 2024 Nov.

DOI:10.1002/pld3.70017
PMID:39553386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11565445/
Abstract

RNA interference (RNAi) is a crucial mechanism in immunity against infectious microbes through the action of DICER-LIKE (DCL) and ARGONAUTE (AGO) proteins. In the case of the taxonomically diverse fungal pathogen and the oomycete , plant DCL and AGO proteins have proven roles as negative regulators of immunity, suggesting functional specialization of these proteins. To address this aspect in a broader taxonomic context, we characterized the colonization pattern of an informative set of and loss-of-function mutants in upon infection with a panel of pathogenic microbes with different lifestyles, and a fungal mutualist. Our results revealed that, depending on the interacting pathogen, AGO1 acts as a positive or negative regulator of immunity, while AGO4 functions as a positive regulator. Additionally, AGO2 and AGO10 positively modulated the colonization by a fungal mutualist. Therefore, analyzing the role of RNAi across a broader range of plant-microbe interactions has identified previously unknown functions for AGO proteins. For some pathogen interactions, however, all tested mutants exhibited wild-type-like infection phenotypes, suggesting that the roles of AGO and DCL proteins in these interactions may be more complex to elucidate.

摘要

RNA干扰（RNAi）是通过类Dicer（DCL）蛋白和AGO蛋白的作用抵御传染性微生物的关键免疫机制。就分类学上多样的真菌病原体和卵菌而言，植物DCL蛋白和AGO蛋白已被证明作为免疫的负调节因子发挥作用，这表明这些蛋白具有功能特异性。为了在更广泛的分类学背景下研究这一方面，我们对一组具有信息价值的AGO和DCL功能缺失突变体在被一组具有不同生活方式的致病微生物以及一种真菌共生体感染后的定殖模式进行了表征。我们的结果表明，根据相互作用的病原体不同，AGO1可作为免疫的正调节因子或负调节因子，而AGO4则作为正调节因子发挥作用。此外，AGO2和AGO10对真菌共生体的定殖起正向调节作用。因此，在更广泛的植物-微生物相互作用范围内分析RNAi的作用，已确定了AGO蛋白以前未知的功能。然而，对于某些病原体相互作用，所有测试的突变体均表现出类似野生型的感染表型，这表明AGO和DCL蛋白在这些相互作用中的作用可能更难以阐明。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/567b/11565445/9dcbb1b9ecd1/PLD3-8-e70017-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/567b/11565445/be6efa1b9c41/PLD3-8-e70017-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/567b/11565445/9dcbb1b9ecd1/PLD3-8-e70017-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/567b/11565445/be6efa1b9c41/PLD3-8-e70017-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/567b/11565445/c5c8b5111e4c/PLD3-8-e70017-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/567b/11565445/9dcbb1b9ecd1/PLD3-8-e70017-g005.jpg

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