AnandaKumar Seethakallu Ramachandraiah, Veerapur Veeresh Prabhakar, Roopesh Marulasiddeshwara, Ambika M V, Babitha S, Thippeswamy Boreddy Shivanandappa
Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumakuru - 572 103, Karnataka, India.
Department of Pharmacology, PESU Institute of Pharmacy, PES University, Bangalore, 560100, Karnataka, India.
Heliyon. 2024 Oct 26;10(21):e39774. doi: 10.1016/j.heliyon.2024.e39774. eCollection 2024 Nov 15.
L. is used in Indian Traditional Medicinal system to treat Diabetes.
This study was carried out to evaluate the standardized alcoholic extract of seed (CPS) in Streptozotocin (STZ)-induced diabetic neuropathic and cardiac complications in rodents.
HPLC finger printing profile of CPS was performed to identify the bioactive molecules. Two doses of CPS (100 & 200 mg/kg b.w.) was orally administered daily once for six weeks to streptozotocin (50 mg/kg, i.v.)-induced diabetic rats. Every week intervals hot & cold immersion tests were carried to know the effect of CPS on peripheral neuropathy. In addition, blood glucose, body weight, food and water intake were also monitored. At the end of the study, sciatic nerve conduction velocity, diabetic cardiomyopathy and diabetic cognitive parameters were evaluated. Furthermore, histopathological studies of sciatic nerve and aortic strip were also carried out.
HPLC finger print experiment showed the presence of gallic acid and protocatechuic acid. Administration of CPS for six weeks significantly prevented the development of diabetic peripheral neuropathy (DPN), cardiomyopathy and cognitive dysfunction in diabetic rats. The CPS treated rats displayed prominent (P < 0.001) improvement in motor coordination, muscle grip, locomotor activity and memory in diabetic rats. CPS treatment restored elevated systolic, diastolic, mean arterial blood pressure (MABP), and heart rate to near normal in diabetic condition. These observed beneficial effects were well correlated and justified with histopathological studies. In addition, CPS treatment also exhibited significant (P < 0.001) reduction of loss in body weight, and reduce the water and feed intake throughout the study.
Taken together, the present study provided a good insight in the therapeutic efficacy of seed extract in dealing with diabetic complications. The study also scientifically justifies the ethnomedicinal/traditional claims of the title plant.
在印度传统医学体系中，L. 被用于治疗糖尿病。
本研究旨在评估种子的标准化酒精提取物（CPS）对链脲佐菌素（STZ）诱导的啮齿动物糖尿病神经病变和心脏并发症的影响。
对CPS进行高效液相色谱指纹图谱分析以鉴定生物活性分子。将两剂量的CPS（100和200毫克/千克体重）每日口服一次，连续六周给予链脲佐菌素（50毫克/千克，静脉注射）诱导的糖尿病大鼠。每隔一周进行冷热浸浴试验以了解CPS对周围神经病变的影响。此外，还监测血糖、体重、食物和水的摄入量。在研究结束时，评估坐骨神经传导速度、糖尿病心肌病和糖尿病认知参数。此外，还对坐骨神经和主动脉条进行了组织病理学研究。
高效液相色谱指纹图谱实验表明存在没食子酸和原儿茶酸。给予CPS六周可显著预防糖尿病大鼠糖尿病周围神经病变（DPN）、心肌病和认知功能障碍的发展。CPS治疗的大鼠在糖尿病大鼠的运动协调、肌肉抓握、运动活动和记忆方面表现出显著（P < 0.001）改善。CPS治疗使糖尿病状态下升高的收缩压、舒张压、平均动脉血压（MABP）和心率恢复到接近正常水平。这些观察到的有益效果与组织病理学研究密切相关且合理。此外，在整个研究过程中，CPS治疗还显著（P < 0.001）减轻了体重减轻，并减少了水和食物的摄入量。
综上所述，本研究为种子提取物在处理糖尿病并发症方面的治疗效果提供了很好的见解。该研究还从科学上证明了标题植物的民族医学/传统说法的合理性。
