小麦矮化基因定位及株高突变等位基因鉴定

Mapping of dwarfing gene and identification of mutant allele on plant height in wheat.

作者信息

Xie Xiaomei, Zhang Yang, Xu Le, Xiong Hongchun, Xie Yongdun, Zhao Linshu, Gu Jiayu, Li Huiyuan, Zhang Jinfeng, Ding Yuping, Zhao Shirong, Guo Huijun, Liu Luxiang

机构信息

College of Agriculture, Hubei Center for Collaborative Innovation of Grain Industry, Yangtze University, Jingzhou, 434025 China.

State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.

出版信息

Mol Breed. 2024 Nov 15;44(11):79. doi: 10.1007/s11032-024-01515-3. eCollection 2024 Nov.

DOI:10.1007/s11032-024-01515-3

PMID:39553699

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11568159/

Abstract

UNLABELLED

Plant height is one of the most critical factors influencing wheat plant architecture, and the application of Green Revolution genes has led to a reduction in plant height and an increase in yield. Discovering new dwarfing genes and alleles can contribute to enhance the genetic diversity of wheat. Here we obtained an EMS induced dwarf wheat mutant with increased grain weight, which exhibited a reduction in plant height ranging from 46.47% to 49.40%, and its cell length was shorter. The mutant was sensitive to exogenous gibberellin, but its sensitivity was lower than that of its wild type. Genetic analysis on plant height and gene mapping located the target region to a 4.07 cM interval on chr. 4AL. Within this interval, we identified a co-segregated mutation in , which is a novel allele of the Green Revolution gene . We also found large fragment inversions in the genetic map of the mutant. The novel allele diversifies natural allelic variations and could be utilized in future wheat improvement. Furthermore, we demonstrated that chemical mutagen treatment led to large fragment inversion.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s11032-024-01515-3.

摘要

未标注

株高是影响小麦株型的最关键因素之一，绿色革命基因的应用导致了株高降低和产量增加。发现新的矮化基因和等位基因有助于提高小麦的遗传多样性。在此，我们获得了一个经甲基磺酸乙酯（EMS）诱变的矮化小麦突变体，其粒重增加，株高降低了46.47%至49.40%，且细胞长度较短。该突变体对外源赤霉素敏感，但其敏感性低于野生型。对株高进行遗传分析并基因定位，将目标区域定位到4AL染色体上4.07厘摩的区间。在此区间内，我们在中鉴定出一个共分离突变，它是绿色革命基因的一个新等位基因。我们还在突变体的遗传图谱中发现了大片段倒位。这个新等位基因使自然等位变异多样化，可用于未来的小麦改良。此外，我们证明化学诱变处理导致了大片段倒位。