Men Yihan, Lu Shan, Li Ling, Wu Chenran, Sun Nannan, Huang Yanju, Yasir Tauqeer Ahmad, Yang Yang, Wang Changhai, Gao Xuefei, Lin Huailong, Zotova Lyudmila, Serikbay Dauren, Liu Yangbin, Yin Yongan, Zeng Chaowu, Hu Yin-Gang, Li Jianjiang, Chen Liang
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production and Collage of Agronomy, Northwest A&F University, Yangling, 712100 Shaanxi China.
Department of Agronomy, Bahauddin Zakariya University, Multan, 60800 Punjab Pakistan.
Mol Breed. 2024 Nov 13;44(11):78. doi: 10.1007/s11032-024-01520-6. eCollection 2024 Nov.
Coleoptile length, in wheat, is a significant agronomic trait impacting yield by facilitating the successful establishment of seedlings. In arid regions, varieties possessing longer coleoptile can evade harsh conditions by deep sowing, paving the way for improved yield. However, the study of genes involved in coleoptile development is insufficient. In this study, a high-density 660 K SNP array was used for genome-wide association study (GWAS) on coleoptile length in 150 wheat varieties. The findings revealed the detection of 353 significantly associated SNPs across all environments. The integration of linkage disequilibrium analysis and haplotype analysis mined 23 core QTLs capable responsible for the stable regulating coleoptile length in wheat. In wheat varieties characterized by extended coleoptile length, 6,600, 11,524, and 6,059 genes were found to be differentially expressed at three distinct developmental stages within the coleoptile, respectively. Through GWAS, gene expression levels, and functional annotation, we concluded the identification of two candidate genes (, ) regulating wheat coleoptile length. By employing WGCNA and protein interactions prediction, discovered that the 19 genes were found to interact with candidate genes and participate in plant hormone metabolism and signaling, cell elongation or proliferation, which collectively contributing to coleoptile elongation. Additionally, two KASP markers were developed which can be used in breeding. These results offer a basis for understanding the genetic regulatory network responsible for wheat coleoptile length formation. The QTLs and candidate genes identified in this study can be further utilized for genetic improvement of wheat coleoptile length.
The online version contains supplementary material available at 10.1007/s11032-024-01520-6.
在小麦中，胚芽鞘长度是一个重要的农艺性状，通过促进幼苗的成功建立来影响产量。在干旱地区，具有较长胚芽鞘的品种可以通过深播来避开恶劣条件，为提高产量铺平道路。然而，对参与胚芽鞘发育的基因的研究还不够充分。在本研究中，使用高密度660K SNP阵列对150个小麦品种的胚芽鞘长度进行全基因组关联研究（GWAS）。研究结果显示在所有环境中检测到353个显著相关的SNP。连锁不平衡分析和单倍型分析相结合挖掘出23个能够稳定调节小麦胚芽鞘长度的核心QTL。在胚芽鞘长度延长的小麦品种中，分别在胚芽鞘的三个不同发育阶段发现6600、11524和6059个基因差异表达。通过GWAS、基因表达水平和功能注释，我们鉴定出两个调控小麦胚芽鞘长度的候选基因（，）。通过应用WGCNA和蛋白质相互作用预测，发现19个基因与候选基因相互作用并参与植物激素代谢和信号传导、细胞伸长或增殖，共同促进胚芽鞘伸长。此外，开发了两个可用于育种的KASP标记。这些结果为理解负责小麦胚芽鞘长度形成的遗传调控网络提供了基础。本研究中鉴定出的QTL和候选基因可进一步用于小麦胚芽鞘长度的遗传改良。
在线版本包含可在10.1007/s11032-024-01520-6获取的补充材料。
