Khalid Arooj, Dremin Viktor, El-Tamer Ayman, Surnina Maria, Lancelot Celine, Rafailov Edik, Sokolovski Sergei
AIPT, College of Engineering and Physical Sciences, Aston University, B4 7 PH Birmingham, UK.
Laser nanoFab GmbH, D-30419 Hannover, Germany.
Biomed Opt Express. 2024 Oct 10;15(11):6299-6312. doi: 10.1364/BOE.510610. eCollection 2024 Nov 1.
The 3D structure of native human skin is fundamental for studying skin health, diseases, wound healing, and for testing the safety of skin care products, as well as personalized treatments for skin conditions. Tissue regeneration, driven by tissue engineering, often involves creating full-thickness skin equivalents (FSE), which are widely used for developing both healthy and diseased skin models. In this study, we utilized human skin cell lines to create FSE. We designed high-resolution 3D scaffolds to support the growth and maturation of these skin models. Additionally, we developed and validated a cost-effective, custom-built system combining fluorescence spectroscopy (FS) and optical coherence tomography (OCT) for non-destructive analysis of the metabolism and morphology of 3D FSEs. This system proved highly sensitive in detecting fluorescence from key metabolic co-enzymes (NADH and FAD) in solutions and cell suspensions, while OCT provided adequate resolution to observe the morphology of FSEs. As a result, both the 3D FSE model and the dual-mode optical system hold significant potential for use in 3D bioprinting of biological tissues, as well as in the development of cosmetics, drugs, and in monitoring their maturation over time.
天然人类皮肤的三维结构对于研究皮肤健康、疾病、伤口愈合以及测试护肤品安全性和针对皮肤状况的个性化治疗至关重要。由组织工程驱动的组织再生通常涉及创建全层皮肤等效物（FSE），其广泛用于开发健康和患病皮肤模型。在本研究中，我们利用人类皮肤细胞系创建FSE。我们设计了高分辨率三维支架以支持这些皮肤模型的生长和成熟。此外，我们开发并验证了一种经济高效的定制系统，该系统结合了荧光光谱（FS）和光学相干断层扫描（OCT），用于对三维FSE的代谢和形态进行无损分析。该系统在检测溶液和细胞悬液中关键代谢辅酶（NADH和FAD）的荧光方面证明高度灵敏，而OCT提供了足够的分辨率来观察FSE的形态。因此，三维FSE模型和双模式光学系统在生物组织的三维生物打印以及化妆品、药物开发和监测其随时间成熟方面都具有巨大的应用潜力。
