Biochemical characterization of mulberry ( spp.) genotypes from the Türkiye (Hizan, Bitlis): A comprehensive analysis of fruit properties and bioactive compounds.

The present study was carried out to assess the fruit traits and phytochemical properties of 39 mulberry genotypes [ L. (16 white mulberry), L. (11 red mulberry), and L. (12 black mulberry)] selected in Bitlis province (Hizan) of Türkiye. Approximately, 1 kg fruit were hand-harvested from each genotype. The fruit size, fruit color, pH, titratable acidity, soluble solids content (SSC), vitamin C, total phenolics, total flavonoids, antioxidant activity, and individual phenolic compounds were determined. The fruit weight varied between 1.80 and 5.84 g in black mulberry, 1.22 and 4.18 g in red mulberry, and 1.29 and 3.10 g in white mulberry. In white mulberry, SSC was determined to be between 19.00% and 38.86%, and in black and red mulberry it was between 11.86% and 21.90% and 12.60% and 18.90%, respectively. The titratable acidity rate, which varied depending on the species, was lower in white mulberry, but the highest acidity rate was recorded in red mulberry. Vitamin C was determined as 33.13 mg 100 g in red mulberry, 24.10 mg 100 g in black mulberry, and 14.03 mg 100 g in white mulberry. Total phenolics in mulberries varied depending on the species, and the red mulberry fruit contained higher phenolic substances, followed by black and white mulberries. The total flavonoids varied between 0.27 and 7.83 g QE kg, whereas the flavonoids varied depending on the species and genotype. The highest values in terms of bioactive compounds were recorded in fruits of the 13HZN23 and 37 genotypes. Black and red mulberry fruit were found to have higher levels of individual phenolic content than the white mulberrry fruit.