Wang Junyi, Xu Peng, Liu Xinhua, Cao Chunxin, Sheng Yingkun, Wang Jianfeng
Life Sciences Zhejiang Normal University Jinhua China.
Hangzhou Normal University of Basic Medicine Hangzhou China.
Food Sci Nutr. 2024 Jun 14;12(9):6284-6297. doi: 10.1002/fsn3.4266. eCollection 2024 Sep.
Sweet pepper, a globally commercialized horticultural crop, has been demonstrated to impede fat accumulation, but its mechanism remains incompletely understood. This study was designed to explore the potential mechanism of sweet pepper in reducing fat accumulation in through RNA-seq and metabolome analysis. A total of 22 metabolites were identified from sweet pepper by UHPLC-ESI-TOF-MS analysis. In vivo, sweet pepper significantly inhibited α-glucosidase activity and reduced the levels of glucose, triglycerides (TG), total cholesterol (TC), and the area stained with oil red O. Additionally, it increased body length and the number of head swings in compared to the control group. The KEGG enrichment analysis revealed significant enrichment of the biosynthesis of unsaturated fatty acids signaling pathway among the differentially expressed genes and metabolites. Furthermore, the mRNA levels of sterol regulatory element-binding proteins (SREBPs) ortholog SBP-1, as well as the stearyl CoA desaturase-1 (SCD1), including , , and , were significantly decreased after treatment with sweet pepper. Collectively, sweet pepper effectively reduces fat accumulation, which is probably related to downregulating the SREBP-SCD axis, offering new insights for future functional food development.
甜椒是一种全球商业化的园艺作物，已被证明可抑制脂肪积累，但其机制仍未完全了解。本研究旨在通过RNA测序和代谢组分析探索甜椒减少脂肪积累的潜在机制。通过超高效液相色谱-电喷雾电离-飞行时间质谱分析从甜椒中鉴定出总共22种代谢物。在体内，甜椒显著抑制α-葡萄糖苷酶活性，并降低葡萄糖、甘油三酯（TG）、总胆固醇（TC）水平以及油红O染色面积。此外，与对照组相比，甜椒增加了体长和头部摆动次数。KEGG富集分析显示，差异表达基因和代谢物中不饱和脂肪酸信号通路的生物合成显著富集。此外，用甜椒处理后，固醇调节元件结合蛋白（SREBPs）直系同源物SBP-1以及硬脂酰辅酶A去饱和酶-1（SCD1）（包括、和）的mRNA水平显著降低。总体而言,甜椒可有效减少脂肪积累，这可能与下调SREBP-SCD轴有关，为未来功能性食品的开发提供了新的见解。
