Dou Lei, Zhang Zimu, Yang Wenqing, Chen Yaobing, Luo Kai, Kan Jianquan
College of Biological and Food Engineering Hubei Minzu University Enshi China.
College of Food Science Southwest University Chongqing China.
Food Sci Nutr. 2024 Jul 10;12(9):6206-6225. doi: 10.1002/fsn3.4325. eCollection 2024 Sep.
In this study, peptides were isolated and purified from Maxim. cake meal for the first time, with the aim of discovering peptides with excellent antioxidant properties. Peptides were isolated and purified from the cake meal using ultrafiltration and dextran gel chromatography. Fractions with significant antioxidant activity were identified by mass spectrometry (MS) and the peptides were screened and characterized using techniques, such as network pharmacology and molecular docking. The results showed that the CIPs-I-F2 fractions possessed excellent antioxidant activities, and a total of seven peptides were screened, with the main targets of action including serine/threonine-protein kinase AKT (AKT1), signal transducer and activator of transcription 3 (STAT3), and matrix metalloproteinase 9 (MMP9), among which ISKPTWADF had the highest total binding energy to the target. ISKPTWADF was synthesized in vitro by solid-phase synthesis and showed a dose-dependent protective effect against the hydrogen peroxide (HO)-induced oxidative damage model in human hepatocellular carcinoma HepG2 cells, with its main active site on the tryptophan indole ring at position 52N-127H.
在本研究中，首次从 Maxim. 蛋糕粉中分离纯化出肽，目的是发现具有优异抗氧化性能的肽。使用超滤和葡聚糖凝胶色谱法从蛋糕粉中分离纯化肽。通过质谱（MS）鉴定具有显著抗氧化活性的馏分，并使用网络药理学和分子对接等技术对肽进行筛选和表征。结果表明，CIPs-I-F2 馏分具有优异的抗氧化活性，共筛选出七种肽，主要作用靶点包括丝氨酸/苏氨酸蛋白激酶 AKT（AKT1）、信号转导和转录激活因子 3（STAT3）以及基质金属蛋白酶 9（MMP9），其中 ISKPTWADF 与靶点的总结合能最高。ISKPTWADF 通过固相合成法在体外合成，并对人肝癌 HepG2 细胞中过氧化氢（HO）诱导的氧化损伤模型表现出剂量依赖性保护作用，其主要活性位点位于 52N - 127H 位色氨酸吲哚环上。
