Wang Haoyu, Huang Mingzhu, Yang Shaofan, Xu Jiameng, Li Jin, Qin Han, Liang Shanshan, Teng Teng, Yang Chuanyan, Gong Mingyue, He Yong, Li Xingyi, Wang Huiquan, Liao Xiang, Chen Xiaowei, Yang Zhiqi, Zhang Kuan
Third Military Medical University, Brain Research Center and State Key Laboratory of Trauma and Chemical Poisoning, Chongqing, China.
Chongqing University, College of Bioengineering, Chongqing, China.
Neurophotonics. 2024 Oct;11(4):045010. doi: 10.1117/1.NPh.11.4.045010. Epub 2024 Nov 15.
Diverse behaviors rely on coordinated activity and multi-regional functional connectivity within astrocyte-neuronal networks. However, current techniques for simultaneously measuring astrocytic and neuronal activities across multiple brain regions during behaviors remain limited.
We propose a multi-fiber solution that can simultaneously record activities of astrocyte-neuronal networks across multiple regions during behaviors.
We employed cell-specific dual-color genetically encoded calcium indicators (GECIs) and multi-fiber photometry to simultaneously measure astrocytic and neuronal Ca transients across multiple brain regions in freely behaving animals.
Our findings demonstrate that both movements and sensory stimuli induce synchronized and highly correlated Ca transients in astrocytes and neurons of freely behaving mice. In addition, we recorded astrocytic and neuronal Ca transients from multiple brain regions during mouse behaviors. Our observations reveal heightened synchronization of astrocytic and neuronal Ca transients across different brain regions during movements or sensory stimuli, indicating enhanced functional connectivity within brain-wide astrocyte-neuronal networks.
Multi-fiber photometry, combined with cell-specific dual-color GECIs, represents a powerful approach for investigating astrocytic and neuronal activities across different brain regions during behaviors. This technique serves as a versatile tool for analyzing the multi-regional functional connectivity map of astrocyte-neuronal networks associated with specific behaviors.
多种行为依赖于星形胶质细胞 - 神经元网络内的协同活动和多区域功能连接。然而，目前在行为过程中同时测量多个脑区星形胶质细胞和神经元活动的技术仍然有限。
我们提出一种多光纤解决方案，该方案能够在行为过程中同时记录多个区域的星形胶质细胞 - 神经元网络活动。
我们采用细胞特异性双色基因编码钙指示剂（GECIs）和多光纤光度法，在自由活动的动物中同时测量多个脑区的星形胶质细胞和神经元钙瞬变。
我们的研究结果表明，运动和感觉刺激均能在自由活动小鼠的星形胶质细胞和神经元中诱导同步且高度相关的钙瞬变。此外，我们在小鼠行为过程中记录了多个脑区的星形胶质细胞和神经元钙瞬变。我们的观察结果显示，在运动或感觉刺激期间，不同脑区的星形胶质细胞和神经元钙瞬变同步性增强，这表明全脑星形胶质细胞 - 神经元网络内的功能连接增强。
多光纤光度法与细胞特异性双色GECIs相结合，是研究行为过程中不同脑区星形胶质细胞和神经元活动的有力方法。该技术是分析与特定行为相关的星形胶质细胞 - 神经元网络多区域功能连接图谱的通用工具。
