Chowdhury Moshfiq-Us-Saleheen, Roy Sutirtha, Aryal Krishna Prasad, Leung Henry, Pandey Richa
Department of Electrical and Software Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
Department of Biomedical Engineering, University of Calgary, Calgary T2N 1N4, Alberta, Canada.
ACS Mater Au. 2024 Jul 23;4(6):592-603. doi: 10.1021/acsmaterialsau.4c00033. eCollection 2024 Nov 13.
Advancements in wearable technology have enabled noninvasive health monitoring using biosensors. This research focuses on developing a textile-based sweat glucose sensor using commercially available conductive textiles, evading the complexity of traditional fabrication methods. A comparative analysis of three low-cost conductive textiles, Adafruit 1364, 1167, and 4762, has been conducted for electrochemical glucose detection with glucose-specific enzymes such as glucose oxidase (GOx) and glucose dehydrogenase (GDH). Adafruit 1364 outperformed others in morphological, electrochemical, and wearable properties. Cyclic voltammetry shows that Adafruit 1364 and 4762 effectively detect glucose at the potential of 0.23 and 0.08 V using glucose oxidase and 0.1 and 0.08 V using glucose dehydrogenase enzymes, respectively. Furthermore, chronoamperometry has been conducted to confirm the presence of glucose at 1 μM concentration. Differential pulse voltammetry was conducted to assess the sensitivity of the Adafruit 1364 fabric electrode using glucose solutions with concentrations of 0.05, 0.15, 0.25, and 0.5 mM. The electrode immobilized with GOx showed a sensitivity of 0.005 μA μM and a limit of detection (LOD) of 41.3 μM, while the electrode immobilized with GDH exhibited a sensitivity of 0.0019 μA μM and an LOD of 63.1 μM. The study also highlighted the reproducibility, effect of interferents, and advantageous wearable properties of these sensors.
可穿戴技术的进步使得使用生物传感器进行无创健康监测成为可能。本研究专注于利用市售导电纺织品开发一种基于纺织品的汗液葡萄糖传感器，避免了传统制造方法的复杂性。对三种低成本导电纺织品Adafruit 1364、1167和4762进行了比较分析，以使用葡萄糖氧化酶（GOx）和葡萄糖脱氢酶（GDH）等葡萄糖特异性酶进行电化学葡萄糖检测。Adafruit 1364在形态、电化学和可穿戴性能方面表现优于其他产品。循环伏安法表明，Adafruit 1364和4762分别使用葡萄糖氧化酶时在0.23 V和0.08 V的电位下以及使用葡萄糖脱氢酶时在0.1 V和0.08 V的电位下能有效检测葡萄糖。此外，进行了计时电流法以确认1 μM浓度葡萄糖的存在。使用浓度为0.05、0.15、0.25和0.5 mM的葡萄糖溶液进行差分脉冲伏安法，以评估Adafruit 1364织物电极的灵敏度。固定有GOx的电极显示出0.005 μA/μM的灵敏度和41.3 μM的检测限（LOD），而固定有GDH的电极表现出0.0019 μA/μM的灵敏度和63.1 μM的LOD。该研究还强调了这些传感器的可重复性、干扰物的影响以及有利的可穿戴性能。
