Lu Lu, Han Chunle, Wang Miao, Du Huanqing, Chen Ning, Gao Mengya, Wang Na, Qi Dongli, Bai Wei, Yin Jianxin, Dong Fengwei, Li Tianshi, Ge Xiaohu
Tingo Exosomes Technology Co. Ltd. Tianjin China.
Pekin University Shenzhen Hospital Shenzhen China.
J Extracell Biol. 2024 Nov 15;3(11):e70009. doi: 10.1002/jex2.70009. eCollection 2024 Nov.
Exosomes are cell-derived nanovesicles that play a crucial role in intercellular communication, presenting promising potential as biomarkers and therapeutic agents. Bovine milk exosomes (MK-Exo) show production scalability and cost-effectiveness, offering distinct advantages over cell-derived exosomes. However, exosome storage and transportation are challenging owing to their unstable nature, necessitating preservation at ultralow temperatures. Research findings suggest that freeze-drying could provide a viable solution; however, different sources of exosomes may require specific protocols. In this study, we aimed to successfully isolate high-purity MK-Exo and develop a specialized freeze-drying and lyophilization method for improved long-term preservation of MK-Exo. Specifically, the stability of the lyophilized MK-Exo was evaluated using storage stability tests. Notably, lyophilized MK-Exo remained stable for at least 3 months under high temperature of 50°C and for at least 24 months under low temperatures of 2°C-8°C, preserving their physicochemical properties and biological activity. Conclusively, these findings provide a potential solution for ambient-temperature transportation of MK-Exo, facilitating their industrial-scale production.
外泌体是细胞衍生的纳米囊泡，在细胞间通讯中发挥着关键作用，作为生物标志物和治疗剂具有广阔的应用前景。牛乳外泌体（MK-Exo）具有生产可扩展性和成本效益，与细胞衍生的外泌体相比具有明显优势。然而，由于外泌体性质不稳定，其储存和运输具有挑战性，需要在超低温下保存。研究结果表明，冷冻干燥可能是一种可行的解决方案；然而，不同来源的外泌体可能需要特定的方案。在本研究中，我们旨在成功分离高纯度的MK-Exo，并开发一种专门的冷冻干燥和冻干方法，以改善MK-Exo的长期保存。具体而言，通过储存稳定性测试评估冻干MK-Exo的稳定性。值得注意的是，冻干的MK-Exo在50°C高温下至少稳定3个月，在2°C-8°C低温下至少稳定24个月，保持其物理化学性质和生物活性。总之，这些发现为MK-Exo的常温运输提供了一种潜在的解决方案，促进了其工业化规模生产。
