Magno Luiz Alexandre Viana, Pinto Sofia Helena Dias Borges, Pacheco Ailla, Rosa Daniela Valadão Freitas, Gubert Priscila, Romano-Silva Marco Aurélio
Programa de Pós-Graduação em Ciências da Saúde (PPGCS), Faculdade Ciências Médicas de Minas Gerais (FCMMG), Alameda Ezequiel Dias, N° 275, Centro, 30130-110 Belo Horizonte, Minas Gerais, Brazil.
INCT em Neurotecnologia Responsável (INCT-NeurotecR), Avenida Alfredo Balena N° 190, Santa Efigênia, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
Toxicol Res (Camb). 2024 Nov 15;13(6):tfae187. doi: 10.1093/toxres/tfae187. eCollection 2024 Dec.
Although dysfunctional Ca signaling can trigger biochemical reactions that lead to cell death, the role of calcium-binding proteins (CBPs) in this process is still a topic of debate. Neuronal calcium sensor 1 (NCS-1) is a CBP that is highly conserved and has been shown to increase cell survival against various types of injuries. As such, we hypothesized that NCS-1 could also be a stress-responsive protein with potential effects on survival and longevity. To explore this possibility, we conducted experiments to examine how mutant nematodes fared under three different stress conditions: hyperosmotic, thermal, and chemical oxidant challenges. Our results showed that while the lack of NCS-1 had no effect on survival responses to hyperosmotic and thermal stresses, worms demonstrated remarkable resistance to the oxidant paraquat in a dose-dependent manner. Based on these findings, we conclude that may employ adaptive mechanisms in the absence of NCS-1 to survive specific oxidative stress stimuli.
尽管功能失调的钙信号传导可触发导致细胞死亡的生化反应，但钙结合蛋白（CBPs）在此过程中的作用仍是一个有争议的话题。神经元钙传感器1（NCS-1）是一种高度保守的钙结合蛋白，已被证明可提高细胞对各种类型损伤的存活率。因此，我们推测NCS-1也可能是一种应激反应蛋白，对生存和寿命具有潜在影响。为了探索这种可能性，我们进行了实验，以研究突变线虫在三种不同应激条件下的表现：高渗、热和化学氧化剂挑战。我们的结果表明，虽然缺乏NCS-1对高渗和热应激的存活反应没有影响，但线虫对氧化剂百草枯表现出显著的剂量依赖性抗性。基于这些发现，我们得出结论，在没有NCS-1的情况下，线虫可能采用适应性机制来在特定的氧化应激刺激下存活。
