• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

金属薄膜表面形貌对铂膜表面辅助激光解吸/电离质谱中离子产率的影响

Effect of the Surface Morphology of a Metal Film on Ion Yields in a Platinum-Film Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.

作者信息

Hashimoto Kotaro, Kaneda Kyosuke, Shimazaki Taichi, Kurihashi Chouma, Yamamoto Shuhei, Takata Riko, Nakanishi Shota, Osaka Issey

机构信息

Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama 939-0398, Japan.

KYOCERA Corporation, 6 Takedatobadonocho, Kyoto Fushimi-ku, Kyoto 612-8450, Japan.

出版信息

Mass Spectrom (Tokyo). 2024;13(1):A0154. doi: 10.5702/massspectrometry.A0154. Epub 2024 Nov 12.

DOI:10.5702/massspectrometry.A0154
PMID:39555378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11565274/
Abstract

Matrix-assisted laser desorption ionization (MALDI) and surface-assisted laser desorption ionization (SALDI) mass spectrometry (MS), which can detect biomolecules and polymers, are widely used in biochemistry and material science. Some compounds that are difficult to ionize using MALDI can be ionized using SALDI. However, it is difficult to obtain high ion yields using SALDI/MS. In this study, a fabricated platinum (Pt) film with nanostructures on the sample surface using a sputtering method was evaluated to determine the optimal metal film for ion yield in SALDI. The surface morphology of the Pt film was analyzed using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray reflectivity (XRR), and ultraviolet-visible-near-infrared (UV-Vis-NIR) reflectance spectroscopy. The SEM, AFM, and TEM images of the Pt film showed the deposited metal film giving high ion yields of polyethylene glycol (PEG) in SALDI/MS with a Pt film (Pt-SALDI) that had a rough surface. The densities and reflectivity of films were analyzed by XRR and UV-Vis-NIR. The higher ion yields of PEG were obtained by Pt-SALDI with the Pt films with lower densities and reflectivity. This indicates that the deposition conditions for the Pt films significantly improved the ion yield in Pt-SALDI/MS. The Pt-SALDI has ionization capabilities different from those of MALDI. Therefore, optimization of Pt film for SALDI/MS and the MS imaging allows more compounds to be detected with higher sensitivity.

摘要

基质辅助激光解吸电离（MALDI）和表面辅助激光解吸电离（SALDI）质谱（MS）能够检测生物分子和聚合物，在生物化学和材料科学中被广泛应用。一些难以用MALDI电离的化合物可用SALDI电离。然而，使用SALDI/MS很难获得高离子产率。在本研究中，对通过溅射法在样品表面制备的具有纳米结构的铂（Pt）薄膜进行了评估，以确定SALDI中实现离子产率的最佳金属薄膜。使用扫描电子显微镜（SEM）、原子力显微镜（AFM）、透射电子显微镜（TEM）、X射线反射率（XRR）和紫外-可见-近红外（UV-Vis-NIR）反射光谱对Pt薄膜的表面形态进行了分析。Pt薄膜的SEM、AFM和TEM图像显示，在SALDI/MS中，具有粗糙表面的Pt薄膜（Pt-SALDI）对聚乙二醇（PEG）具有较高的离子产率。通过XRR和UV-Vis-NIR分析了薄膜的密度和反射率。密度和反射率较低的Pt薄膜的Pt-SALDI对PEG具有更高的离子产率。这表明Pt薄膜的沉积条件显著提高了Pt-SALDI/MS中的离子产率。Pt-SALDI具有与MALDI不同的电离能力。因此，对SALDI/MS的Pt薄膜进行优化以及进行质谱成像可使更多化合物以更高的灵敏度被检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/74eb335b6a37/massspectrometry-13-1-A0154-figure06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/6a11c225156c/massspectrometry-13-1-A0154-figure01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/ac66df9f363a/massspectrometry-13-1-A0154-figure02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/90867e2747ac/massspectrometry-13-1-A0154-figure03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/b2df00da40fb/massspectrometry-13-1-A0154-figure04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/bdce1fc4b0ce/massspectrometry-13-1-A0154-figure05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/74eb335b6a37/massspectrometry-13-1-A0154-figure06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/6a11c225156c/massspectrometry-13-1-A0154-figure01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/ac66df9f363a/massspectrometry-13-1-A0154-figure02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/90867e2747ac/massspectrometry-13-1-A0154-figure03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/b2df00da40fb/massspectrometry-13-1-A0154-figure04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/bdce1fc4b0ce/massspectrometry-13-1-A0154-figure05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/109c/11565274/74eb335b6a37/massspectrometry-13-1-A0154-figure06.jpg

相似文献

1
Effect of the Surface Morphology of a Metal Film on Ion Yields in a Platinum-Film Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.金属薄膜表面形貌对铂膜表面辅助激光解吸/电离质谱中离子产率的影响
Mass Spectrom (Tokyo). 2024;13(1):A0154. doi: 10.5702/massspectrometry.A0154. Epub 2024 Nov 12.
2
Simultaneous detection of phosphatidylcholines and glycerolipids using matrix-enhanced surface-assisted laser desorption/ionization-mass spectrometry with sputter-deposited platinum film.使用溅射沉积铂膜的基质增强表面辅助激光解吸/电离质谱法同时检测磷脂酰胆碱和甘油脂。
J Mass Spectrom. 2015 Nov;50(11):1264-9. doi: 10.1002/jms.3700.
3
Direct Imaging Mass Spectrometry of Plant Leaves Using Surface-assisted Laser Desorption/Ionization with Sputter-deposited Platinum Film.使用溅射沉积铂膜的表面辅助激光解吸/电离对植物叶片进行直接成像质谱分析。
Anal Sci. 2016;32(5):587-91. doi: 10.2116/analsci.32.587.
4
Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition.使用通过雾状化学气相沉积形成金属膜的表面辅助激光解吸/电离质谱法对磷脂酰胆碱和二酰基甘油进行成像分析
Mass Spectrom (Tokyo). 2023;12(1):A0135. doi: 10.5702/massspectrometry.A0135. Epub 2023 Nov 25.
5
Analyses of functional polymer-modified nanoparticles for protein sensing by surface-assisted laser desorption/ionization mass spectrometry coupled with HgTe nanomatrices.通过表面辅助激光解吸/电离质谱与 HgTe 纳米基质联用分析功能聚合物修饰纳米颗粒用于蛋白质传感。
Colloids Surf B Biointerfaces. 2015 Jun 1;130:157-63. doi: 10.1016/j.colsurfb.2015.04.001. Epub 2015 Apr 7.
6
Platinum vapor deposition surface-assisted laser desorption/ionization for imaging mass spectrometry of small molecules.铂蒸气沉积表面辅助激光解吸/电离用于小分子的成像质谱分析。
Rapid Commun Mass Spectrom. 2012 Aug 30;26(16):1849-58. doi: 10.1002/rcm.6301.
7
Molecularly imprinted polymers based on magnetic metal-organic frameworks for surface-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and simultaneous luteolin enrichment.基于磁性金属有机骨架的分子印迹聚合物用于表面辅助激光解吸/电离飞行时间质谱分析和同时富集木樨草素。
J Chromatogr A. 2022 Aug 16;1678:463377. doi: 10.1016/j.chroma.2022.463377. Epub 2022 Jul 26.
8
Metabolite imaging using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS).使用基质增强表面辅助激光解吸/电离质谱法（ME-SALDI-MS）进行代谢物成像。
J Am Soc Mass Spectrom. 2009 Jan;20(1):80-8. doi: 10.1016/j.jasms.2008.09.011. Epub 2008 Sep 20.
9
Functionalized pyrolytic highly oriented graphite polymer film for surface-assisted laser desorption/ionization mass spectrometry in environmental analysis.用于环境分析中表面辅助激光解吸/电离质谱的功能化热解高取向石墨聚合物薄膜
Rapid Commun Mass Spectrom. 2009 Oct 30;23(20):3323-32. doi: 10.1002/rcm.4254.
10
Matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) for mass spectrometry imaging of small molecules.用于小分子质谱成像的基质增强表面辅助激光解吸/电离质谱（ME-SALDI-MS）
Methods Mol Biol. 2015;1203:175-84. doi: 10.1007/978-1-4939-1357-2_17.

引用本文的文献

1
Direct Additive Detection in Polymer Films via Platinum-Assisted SALDI Mass Spectrometry Imaging.通过铂辅助表面增强激光解吸电离质谱成像在聚合物薄膜中进行直接添加剂检测。
Mass Spectrom (Tokyo). 2024;13(1):A0162. doi: 10.5702/massspectrometry.A0162. Epub 2024 Dec 17.

本文引用的文献

1
Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition.使用通过雾状化学气相沉积形成金属膜的表面辅助激光解吸/电离质谱法对磷脂酰胆碱和二酰基甘油进行成像分析
Mass Spectrom (Tokyo). 2023;12(1):A0135. doi: 10.5702/massspectrometry.A0135. Epub 2023 Nov 25.
2
Development of an Automatic Ultrasonic Matrix Sprayer for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.自动超声矩阵喷雾器的开发用于基质辅助激光解吸/电离质谱成像。
Anal Chem. 2022 May 3;94(17):6457-6462. doi: 10.1021/acs.analchem.2c00403. Epub 2022 Apr 19.
3
Surface-assisted laser desorption/ionization mass spectrometry imaging: A review.
表面辅助激光解吸/电离质谱成像：综述。
Mass Spectrom Rev. 2022 May;41(3):373-420. doi: 10.1002/mas.21670. Epub 2020 Nov 10.
4
Novel approach to enhance sensitivity in surface-assisted laser desorption/ionization mass spectrometry imaging using deposited organic-inorganic hybrid matrices.使用沉积的有机-无机杂化基质提高表面辅助激光解吸/电离质谱成像灵敏度的新方法。
J Mass Spectrom. 2019 Jul;54(7):612-619. doi: 10.1002/jms.4370.
5
Direct Imaging Mass Spectrometry of Plant Leaves Using Surface-assisted Laser Desorption/Ionization with Sputter-deposited Platinum Film.使用溅射沉积铂膜的表面辅助激光解吸/电离对植物叶片进行直接成像质谱分析。
Anal Sci. 2016;32(5):587-91. doi: 10.2116/analsci.32.587.
6
Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures.利用机器人喷雾器进行微生物培养物的高横向分辨率和质量分辨率基质辅助激光解吸电离傅里叶变换离子回旋共振质谱成像
J Am Soc Mass Spectrom. 2016 Mar;27(3):556-9. doi: 10.1007/s13361-015-1324-6. Epub 2016 Jan 4.
7
Simultaneous detection of phosphatidylcholines and glycerolipids using matrix-enhanced surface-assisted laser desorption/ionization-mass spectrometry with sputter-deposited platinum film.使用溅射沉积铂膜的基质增强表面辅助激光解吸/电离质谱法同时检测磷脂酰胆碱和甘油脂。
J Mass Spectrom. 2015 Nov;50(11):1264-9. doi: 10.1002/jms.3700.
8
Alternative two-step matrix application method for imaging mass spectrometry to avoid tissue shrinkage and improve ionization efficiency.两步式基质辅助激光解吸电离成像质谱法的替代方法，可避免组织收缩并提高离子化效率。
J Mass Spectrom. 2013 Dec;48(12):1285-90. doi: 10.1002/jms.3288.
9
MALDI of synthetic polymers with labile end-groups.具有不稳定端基的合成聚合物的基质辅助激光解吸电离
Mass Spectrom Rev. 2014 Nov-Dec;33(6):523-43. doi: 10.1002/mas.21403. Epub 2013 Nov 27.
10
A new combination MALDI matrix for small molecule analysis: application to imaging mass spectrometry for drugs and metabolites.一种用于小分子分析的新型 MALDI 基质组合：在药物和代谢物的成像质谱中的应用。
Analyst. 2012 Dec 21;137(24):5757-62. doi: 10.1039/c2an35782h.