Zhang Haoran, Guo Haoji, Lin Jiali, He Xianda, Rong Hua, Lin Fan, Wen Xiaobo
College of Marine Sciences South China Agricultural University, Guangzhou 510642, China.
Nansha-South China Agricultural University Fishery Research Institute, Guangzhou 511466, China.
Aquac Nutr. 2024 Sep 19;2024:7867796. doi: 10.1155/2024/7867796. eCollection 2024.
The widespread use of commercial feeds with high-fat content in aquaculture may lead to oxidative stress and inflammation in fish during culture, which may cause changes in fish muscle texture. Therefore, mitigating oxidative stress and inflammation during fish farming holds paramount importance in improving fish muscle quality. In this study, we investigated the effect of different dietary levels of chlorogenic acid (CGA; 0, 100, 200, 400, 800, 1600 mg/kg diet, P1-P6) in commercial diets on the muscle of blackspotted croaker (s) through an 8-week feeding trial in open sea cages. The results showed that high dietary CGA levels (P5-P6) could significantly reduce muscle oxidative stress and inflammation ( < 0.05). Muscle toughness (hardness, chewiness, shear force, and gumminess) improved significantly as CGA levels increased ( < 0.05). It was also observed that the gap of muscle fiber was significantly reduced, while the muscle fiber density was significantly increased with the increase of CGA level ( < 0.05). Notably, dietary CGA also had a significant effect on collagen content in the muscle ( < 0.05), which may also be a crucial factor affecting muscle texture. Furthermore, it was found that the reduction of inflammation and increase of collagen deposition in muscle by dietary CGA may be related to the upregulation of transforming growth factor beta (TGF-) signaling pathway. Finally, it can be concluded that a dietary CGA supplementation of 1173.11 mg/kg is suggested for the aquaculture of , based on the muscle texture quality.
水产养殖中广泛使用高脂肪含量的商业饲料可能会导致养殖过程中鱼类出现氧化应激和炎症，这可能会引起鱼肌肉质地的变化。因此，在养鱼过程中减轻氧化应激和炎症对于提高鱼的肌肉质量至关重要。在本研究中，我们通过在开放式海笼中进行为期8周的投喂试验，研究了商业饲料中不同膳食水平的绿原酸（CGA；0、100、200、400、800、1600毫克/千克饲料，P1 - P6）对黑鲷肌肉的影响。结果表明，高膳食CGA水平（P5 - P6）可显著降低肌肉氧化应激和炎症（P < 0.05）。随着CGA水平的升高，肌肉韧性（硬度、咀嚼性、剪切力和黏性）显著提高（P < 0.05）。还观察到，随着CGA水平的升高，肌纤维间隙显著减小，而肌纤维密度显著增加（P < 0.05）。值得注意的是，膳食CGA对肌肉中的胶原蛋白含量也有显著影响（P < 0.05），这也可能是影响肌肉质地的关键因素。此外，发现膳食CGA对肌肉炎症的减轻和胶原蛋白沉积的增加可能与转化生长因子β（TGF - ）信号通路的上调有关。最后，可以得出结论，基于肌肉质地质量，建议黑鲷水产养殖中膳食CGA补充量为1173.11毫克/千克。
