College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Poult Sci. 2023 Mar;102(3):102479. doi: 10.1016/j.psj.2023.102479. Epub 2023 Jan 6.
This study was conducted to investigate the protective effects of chlorogenic acid (CGA) on broilers subjected to (DQ)-induced oxidative stress. In experiment 1, one hundred and ninety-two male one-day-old Ross 308 broiler chicks were distributed into 4 groups and fed a basal diet supplemented with 0, 250, 500, or 1,000 mg/kg CGA for 21 d. In experiment 2, an equivalent number of male one-day-old chicks were allocated to 4 treatments for a 21-d trial: 1) Control group, normal birds fed a basal diet; 2) DQ group, DQ-challenged birds fed a basal diet; and 3) and 4) CGA-treated groups: DQ-challenged birds fed a basal diet supplemented with 500 or 1,000 mg/kg CGA. The intraperitoneal DQ challenge was performed at 20 d. In experiment 1, CGA administration linearly increased 21-d body weight, and weight gain and feed intake during 1 to 21 d (P < 0.05). CGA linearly and/or quadratically increased total antioxidant capacity, catalase, superoxide dismutase, and glutathione peroxidase activities, elevated glutathione level, and reduced malondialdehyde accumulation in serum, liver, and/or jejunum (P < 0.05). In experiment 2, compared with the control group, DQ challenge reduced body weight ratio (P < 0.05), which was reversed by CGA administration (P < 0.05). DQ challenge increased serum total protein level, aspartate aminotransferase activity, and total bilirubin concentration (P < 0.05), which were normalized when supplementing 500 mg/kg and/or 1,000 mg/kg CGA (P < 0.05). DQ administration elevated hepatic interleukin-1β, tumor necrosis factor-α, and interleukin-6 levels (P < 0.05), and the values of interleukin-1β were normalized to control values when supplementing CGA (P < 0.05). DQ injection decreased serum superoxide dismutase activity, hepatic catalase activity, and serum and hepatic glutathione level, but increased malondialdehyde concentration in serum and liver (P < 0.05), and the values of these parameters (except hepatic catalase activity) were reversed by 500 and/or 1,000 mg/kg CGA. The results suggested that CGA could improve growth performance, alleviate oxidative stress, and ameliorate hepatic inflammation in DQ-challenged broilers.
本研究旨在探讨绿原酸（CGA）对（DQ）诱导的氧化应激肉鸡的保护作用。在实验 1 中，将 192 只 1 日龄雄性罗斯 308 肉鸡分为 4 组，分别饲喂基础日粮，并添加 0、250、500 或 1000mg/kg CGA，为期 21d。在实验 2 中，将相同数量的 1 日龄雄性肉鸡分配到 4 个处理组进行 21d 试验：1）对照组，正常鸡饲喂基础日粮；2）DQ 组，基础日粮饲喂 DQ 应激鸡；3）和 4）CGA 处理组：基础日粮饲喂 DQ 应激鸡，并添加 500 或 1000mg/kg CGA。20d 时进行腹腔内 DQ 挑战。在实验 1 中，CGA 给药呈线性增加 21d 体重、1-21d 体重增加和采食量（P<0.05）。CGA 呈线性和/或二次增加总抗氧化能力、过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性，提高谷胱甘肽水平，降低血清、肝脏和/或空肠丙二醛积累（P<0.05）。在实验 2 中，与对照组相比，DQ 挑战降低了体重比（P<0.05），CGA 给药可逆转这一结果（P<0.05）。DQ 挑战增加了血清总蛋白水平、天冬氨酸氨基转移酶活性和总胆红素浓度（P<0.05），当补充 500mg/kg 和/或 1000mg/kg CGA 时，这些值恢复正常（P<0.05）。DQ 给药增加了肝白细胞介素-1β、肿瘤坏死因子-α和白细胞介素-6 水平（P<0.05），当补充 CGA 时，白细胞介素-1β值恢复到对照值（P<0.05）。DQ 注射降低了血清超氧化物歧化酶活性、肝过氧化氢酶活性以及血清和肝谷胱甘肽水平，但增加了血清和肝脏丙二醛浓度（P<0.05），500 和/或 1000mg/kg CGA 可逆转这些参数（肝过氧化氢酶活性除外）。结果表明，CGA 可改善 DQ 应激肉鸡的生长性能，减轻氧化应激，改善肝炎症。
