Devi Ng Chinglembi, Waikhom Gusheinzed, Singh Soibam Khogen, Das Pronob, Debbarma Sourabh, Debbarma Reshmi, Singh Lukram Sushil, Meinam Martina, Biswas Pradyut, Irungbam Surajkumar
Department of Aquaculture, College of Fisheries, Central Agricultural University, Agartala 799210, Tripura, India.
Krishi Vigyan Kendra, ICAR Research Complex for NEH Region Manipur Centre, Ukhrul 795142, Manipur, India.
Aquac Nutr. 2024 Oct 12;2024:9930544. doi: 10.1155/2024/9930544. eCollection 2024.
Biofloc technology offers a viable choice for the rearing of fish seed by offering a safe and protected habitat for young fish. Nevertheless, it is crucial to establish a standardised stocking density and implement effective ameliorative steps in order to successfully utilise this technology. In this study, a 90-day investigation was conducted to assess the effect of stocking density and dietary vitamin C (Vit C) levels on the growth and immunity of fry (1.03 ± 0.01 g) reared in a biofloc system. Three stocking density groups (5, 10 and 15 fish per 50 L) were established, and each experimental group was supplemented with three levels of Vit C (0, 500 and 1000 mg kg). The highest survival rate was observed in the lowest density group (five fish per 50 L) fed with 1000 mg kg Vit C. A better feed conversion ratio and significantly higher protein efficiency ratio were found in the moderate stocking density group (10 fish per 50 L) fed with 500 mg kg Vit C. Total leukocyte count (TLC), haemoglobin, and packed cell volume improved in Vit C-fed groups. The total erythrocyte count (TEC) increased in groups fed Vit C and stocked at lower densities (5 and 10 fish per 50 L). Total serum protein (TPP) content increased when Vit C was added at a rate of 500 mg kg. Serum glucose and cortisol levels were significantly reduced in Vit C-supplemented groups. Supplementation of Vit C at 500 mg kg resulted in a significantly lower value of malondialdehyde (MDA). Thus, the findings confirm that the incorporation of Vit C in the basal diet promotes the growth and health status of fry reared in the biofloc system at high-density rearing.
生物絮团技术通过为幼鱼提供一个安全且受保护的栖息地，为鱼苗培育提供了一个可行的选择。然而，为了成功利用这项技术，建立标准化的放养密度并采取有效的改善措施至关重要。在本研究中，进行了为期90天的调查，以评估放养密度和日粮维生素C（Vit C）水平对在生物絮团系统中饲养的鱼苗（1.03±0.01克）生长和免疫的影响。设立了三个放养密度组（每50升5、10和15尾鱼），每个实验组补充三种水平的Vit C（0、500和1000毫克/千克）。在投喂1000毫克/千克Vit C的最低密度组（每50升5尾鱼）中观察到最高存活率。在投喂500毫克/千克Vit C的中等放养密度组（每50升10尾鱼）中发现了更好的饲料转化率和显著更高的蛋白质效率比。在投喂Vit C的组中，总白细胞计数（TLC）、血红蛋白和血细胞压积有所改善。在投喂Vit C且放养密度较低（每50升5和10尾鱼）的组中，总红细胞计数（TEC）增加。当以500毫克/千克的速率添加Vit C时，总血清蛋白（TPP）含量增加。在补充Vit C的组中，血清葡萄糖和皮质醇水平显著降低。以500毫克/千克补充Vit C导致丙二醛（MDA）值显著降低。因此，研究结果证实，在基础日粮中添加Vit C可促进在生物絮团系统中高密度饲养的鱼苗的生长和健康状况。
