Habib Syed Sikandar, Saeed Muhammad Qamar, Masud Samrah, Kesbiç Osman Sabri, Ujan Javed Ahmed, Cravana Cristina, Al-Rejaie Salim S, Mohany Mohamed, Fazio Francesco
Department of Zoology, University of Sargodha, Sargodha 40100, Punjab, Pakistan.
Department of Microbiology and Molecular Genetics, Bahauddin Zakariya University Multan, Multan 60800, Punjab, Pakistan.
Aquac Nutr. 2024 Jun 13;2024:7321287. doi: 10.1155/2024/7321287. eCollection 2024.
This study investigates the influence of root powder (WSRP) on different aspects of common carp (), including growth, hematobiochemical parameters, antioxidant status, nonspecific immune response, and resilience to bacterial infections. Over a 60-day period, 180 common carp fingerlings (11.73 ± 0.52 g) were subjected to diets supplemented with increasing WSRP levels (0 (control), 1% (WSRP1), 2.5% (WSRP2.5), and 4% (WSRP4)). Nonspecific immune parameters were evaluated using serum samples collected at intervals of 0, 20, 40, and 60 days. After the feeding trial, the fish underwent experimental challenge with , and relative percentage survival (RPS) was monitored for 14 days. The findings revealed a considerable ( < 0.05) enhancement in growth performance and a decreased feed conversion ratio (FCR) with increasing WSRP supplementation. Additionally, hematological and biochemical profiles exhibited improvements in groups receiving WSRP-enriched diets. Fish serum antioxidant status showed a significant ( < 0.05) increase, as indicated by increased activities of total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in WSRP4. WSRP-supplemented diets led to remarkable enhancements in lysozyme activity ( = 0.001), phagocytic activity ( = 0.002), and RPS ( = 0.003), peaking in WSRP4 at day 60. Furthermore, WSRP2.5 and WSRP4 demonstrated a substantial decrease ( > 0.05) in serum glucose and cortisol levels compared to other groups. In conclusion, WSRP proves valuable for enhancing growth, blood parameters, antioxidant balance, immune response, and infection resistance in common carp, especially at 2.5%-4% dietary supplementation levels. In the future, it will be crucial to study the long-term effects of WSRP supplementation on fish health, as well as its potential for large-scale aquaculture and economic viability in the aquafeed industry.
本研究调查了芜菁根粉（WSRP）对鲤鱼不同方面的影响，包括生长、血液生化参数、抗氧化状态、非特异性免疫反应以及对细菌感染的抵抗力。在60天的时间里，180尾鲤鱼幼鱼（11.73±0.52克）被投喂添加了不同水平WSRP的饲料（0%（对照）、1%（WSRP1）、2.5%（WSRP2.5）和4%（WSRP4））。在第0、20、40和60天采集血清样本，评估非特异性免疫参数。投喂试验结束后，对鱼进行嗜水气单胞菌的攻毒试验，并监测14天的相对存活率（RPS）。研究结果显示，随着WSRP添加量的增加，生长性能显著提高（P<0.05），饲料转化率降低。此外，接受富含WSRP饲料的组的血液学和生化指标有所改善。鱼血清抗氧化状态显著增加（P<0.05），WSRP4组的总抗氧化能力（TAC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和还原型谷胱甘肽（GSH）活性增加。添加WSRP的饲料显著提高了溶菌酶活性（P=0.001）、吞噬活性（P=0.002）和RPS（P=0.003），在第60天WSRP4组达到峰值。此外，与其他组相比，WSRP2.5和WSRP4组的血清葡萄糖和皮质醇水平显著降低（P>0.05）。总之，WSRP被证明对提高鲤鱼的生长、血液参数、抗氧化平衡、免疫反应和抗感染能力具有重要价值，尤其是在饲料中添加2.5%-4%的水平时。未来，研究WSRP添加对鱼类健康的长期影响，以及其在大规模水产养殖中的潜力和水产饲料行业的经济可行性至关重要。
