Sennerstam R, Strömberg J O
Cell Tissue Kinet. 1986 Jan;19(1):57-70. doi: 10.1111/j.1365-2184.1986.tb00715.x.
Analyses of cell-cycle characteristics of the three embryonal carcinoma (EC) cell lines F9, PCC3 N/1 and PCC4 Azal, have been performed. The three lines reflect successive stages in early mouse embryogenesis as regards cell surface antigens and cell-cycle characteristics. In an attempt to understand changes in cell-cycle characteristics occurring during early embryogenesis, the two-random transition probability (TP) model was applied to the EC-cell system--and particularly to the F9 line. By utilizing an intraclonal heterogeneity in intermitotic times found in these EC lines, a growth-regulating point was introduced as a modification of the two-random TP model. The modified model was found to be very useful when demonstrating the cell-cycle growth kinetics of the F9 line. The model is used in an accompanying paper to extend the analysis of cell-cycle characteristics in undifferentiated EC cells.
已对三种胚胎癌（EC）细胞系F9、PCC3 N/1和PCC4 Azal的细胞周期特征进行了分析。就细胞表面抗原和细胞周期特征而言，这三种细胞系反映了小鼠早期胚胎发育的连续阶段。为了了解早期胚胎发育过程中发生的细胞周期特征变化，将双随机转变概率（TP）模型应用于EC细胞系统，尤其是F9细胞系。通过利用这些EC细胞系中发现的有丝分裂间期的克隆内异质性，引入了一个生长调节点作为对双随机TP模型的修正。发现修正后的模型在展示F9细胞系的细胞周期生长动力学时非常有用。该模型在随附论文中用于扩展对未分化EC细胞的细胞周期特征分析。
