Sennerstam R, Strömberg J O
Dev Biol. 1984 May;103(1):221-9. doi: 10.1016/0012-1606(84)90023-x.
Three embryonal carcinoma (EC) cell lines F9, PCC4 Azal, and PCC3 N/1 with a common origin from the transplantable ascites teratoma OTT6050 were analyzed with regard to cell cycle characteristics during exponential growth in the undifferentiated state. The three lines, in the sequence mentioned, have been shown to represent successive stages in early normal mouse embryogenesis from morula to blastocyst, as reflected in their cell surface antigens. In the multipotent lines an increased intraclonal variability in intermitotic times was found when analyzing family trees. In the PCC3 N/1 line the distribution was almost bimodal. The increased intermitotic time variability was found to be represented in the G1 period that varied from less than 1 hr up to about 6 hr in the PCC3 N/1 line, with a mean value of 3.4 hr. The G1 period of the F9 line had a range of only 3.5 hr, with a mean value of 1.5 hr. The S-G2 period was found to be very similar in length in all three lines.
对起源于可移植腹水畸胎瘤OTT6050的三种胚胎癌（EC）细胞系F9、PCC4 Azal和PCC3 N/1在未分化状态下指数生长期间的细胞周期特征进行了分析。如细胞表面抗原所示，上述三种细胞系按提及的顺序代表了正常小鼠胚胎早期从桑椹胚到囊胚的连续阶段。在分析家系树时，发现多能细胞系中细胞分裂间期的克隆内变异性增加。在PCC3 N/1细胞系中，分布几乎呈双峰。细胞分裂间期变异性增加表现为G1期的变化，在PCC3 N/1细胞系中，G1期从不到1小时到约6小时不等，平均值为3.4小时。F9细胞系的G1期范围仅为3.5小时，平均值为1.5小时。发现所有三种细胞系的S-G2期长度非常相似。
