Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 3 Rokietnicka Street, Poznań, 60-806, Poland.
Department of Nucleic Acid Biochemistry, Medical University of Lodz, Lodz, 92-213, Poland.
Sci Rep. 2024 Nov 19;14(1):28562. doi: 10.1038/s41598-024-79479-9.
There is a growing interest and demand for insect-based foods. Edible insects are rich in protein and other nutrients, making them valuable in the daily diet. However, their composition is not yet fully characterised. Therefore, this study aimed to analyse for the first time the qualitative proteome of honeybee (Apis mellifera) drone larvae using sophisticated sample preparation techniques and mass spectrometry. A total of 109 proteins were identified in the larvae. Of these, the largest plurality (38%) were enzymes. In addition, we identified proteins considered to be allergens - the cause of potentially dangerous effects after insect consumption. The results of the analyses may suggest that honeybee larvae are a protein-rich product, with over 100 unique proteins identified based on 1080 peptides. Enzymes indicate intensive development of the larvae. However, as well as nutritious compounds, honeybee larvae contain dangerous allergens. The composition of bee larvae needs to be further tested to make them safe for consumption.
人们对昆虫类食物的兴趣和需求日益增长。食用昆虫富含蛋白质和其他营养物质，因此在日常饮食中具有很高的价值。然而，它们的成分尚未得到充分的描述。因此，本研究首次使用复杂的样品制备技术和质谱法分析了雄蜂幼虫（Apis mellifera）的定性蛋白质组。在幼虫中鉴定出了总共 109 种蛋白质。其中，最大的多态性（38%）是酶。此外，我们还鉴定出了被认为是过敏原的蛋白质 - 这些蛋白质是昆虫食用后可能产生危险影响的原因。分析结果表明，雄蜂幼虫是一种富含蛋白质的产品，基于 1080 个肽段鉴定出了 100 多种独特的蛋白质。酶表明幼虫的发育很旺盛。然而，除了有营养的化合物外，雄蜂幼虫还含有危险的过敏原。需要进一步测试蜜蜂幼虫的组成，以确保它们可以安全食用。
