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制备细胞中间体EAC14的简单方法及其在补体第二成分估计中的应用。

Simple method for preparing the cellular intermediate EAC14, and its use for estimation of the second component of complement.

作者信息

Asghar S S, Out J, van der Helm H J

出版信息

Clin Chem. 1986 Apr;32(4):678-9.

PMID:3955817
Abstract

Sensitized erythrocytes carrying the first and fourth components of complement (EAC14) were prepared by incubating optimally sensitized sheep erythrocytes with normal serum appropriately diluted in Mg2+-free diethylbarbiturate buffer containing Ca2+. EAC14 cells so prepared were found to be suitable for use in estimating the second component of complement (C2) in human serum, and the method is described here.

摘要

通过将最佳致敏的绵羊红细胞与在含Ca2+的无Mg2+二乙巴比妥缓冲液中适当稀释的正常血清孵育，制备携带补体第一和第四成分的致敏红细胞（EAC14）。发现如此制备的EAC14细胞适用于估计人血清中的补体第二成分（C2），本文描述了该方法。

相似文献

1
Simple method for preparing the cellular intermediate EAC14, and its use for estimation of the second component of complement.制备细胞中间体EAC14的简单方法及其在补体第二成分估计中的应用。
Clin Chem. 1986 Apr;32(4):678-9.
2
New methods for the preparation of the cellular intermediate EAC14.制备细胞中间体EAC14的新方法。
J Immunol Methods. 1986 Dec 24;95(2):277-81. doi: 10.1016/0022-1759(86)90416-3.
3
Surface modulation of classical pathway activation: C2 and C3 convertase formation and regulation on sheep, guinea pig, and human erythrocytes.经典途径激活的表面调节：绵羊、豚鼠和人红细胞上C2和C3转化酶的形成与调节
J Immunol. 1983 Jul;131(1):403-8.
4
EAC4 and EAC14 production without purified Ci.在没有纯化的Ci的情况下生产EAC4和EAC14。
J Immunol. 1975 Dec;115(6):1625-30.
5
IgG and complement-mediated tissue damage in the absence of C2: evidence of a functionally active C2-bypass pathway in a guinea pig model.在缺乏C2的情况下IgG和补体介导的组织损伤：豚鼠模型中功能活跃的C2旁路途径的证据
J Immunol. 1999 Sep 15;163(6):3549-58.
6
In vitro inhibition of the classical pathway of human complement by polymyxin B.
Biochem Pharmacol. 1987 Sep 15;36(18):2927-30. doi: 10.1016/0006-2952(87)90204-8.
7
Lysis of sensitized sheep erythrocytes in human sera deficient in the second component of complement.在缺乏补体第二成分的人血清中致敏绵羊红细胞的溶解。
J Immunol. 1989 Oct 1;143(7):2256-61.
8
A new haemolytic assay for the second component of complement (C2) in human serum.一种用于检测人血清中补体第二成分（C2）的新型溶血试验。
Clin Exp Immunol. 1976 Sep;25(3):487-9.
9
A simple one-step hemolytic assay for C2 with C2-deficient human serum.
J Immunol. 1977 Mar;118(3):736-41.
10
Complement inhibitor(s) released by leukocytes. I. Pretreatment of sheep erythrocytes with supernatants of mouse spleen and thymus cells inhibit whole complement activity and C2 utilization.白细胞释放的补体抑制剂。I. 用小鼠脾脏和胸腺细胞的上清液预处理绵羊红细胞可抑制总补体活性和C2利用。
J Immunol. 1975 Oct;115(4):1087-90.

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