Ameen Fuad, Almalki Norah Salem, Alshalan Rawan, Sakayanathan Penislusshiyan
Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Laboratory and Blood Bank Department, King Fahad General Hospital, Second Cluster, Jeddah, Saudi Arabia.
Microsc Res Tech. 2025 Mar;88(3):749-760. doi: 10.1002/jemt.24726. Epub 2024 Nov 18.
Selenium nanoparticles (SeNPs) have garnered significant interest as anticancer and antimicrobial agents. The aqueous extract of medicinal plant Drimia indica leaves (DI-LAE) was used to synthesize SeNPs (DI-SeNPs) that were extensively characterized by UV-visible absorbance, TEM, EDX, XRD, zeta potential measurements, and FTIR. DI-SeNPs exhibited dose-dependent toxicity against the human lung adenocarcinoma cell line (A549; IC of 43.21 μg/mL). DI-SeNPs increased reactive oxygen species (ROS) generation in A549 cells. DI-SeNPs caused cell cycle arrest in the G2/M phase and increased DNA damage in A549 cells, ultimately driving these cells toward apoptosis. DI-SeNPs significantly increased p53 levels, decreasing Akt levels and elevating cleaved caspase 3 levels in A549 cells. Additionally, DI-SeNPs exhibited antimicrobial activity against various bacteria and fungi. These findings suggest that DI-SeNPs possess significant anticancer and antimicrobial properties, mediated through mechanisms involving ROS generation, cell cycle arrest, and apoptosis induction.
硒纳米颗粒（SeNPs）作为抗癌和抗菌剂已引起广泛关注。药用植物印度海葱叶水提取物（DI-LAE）被用于合成硒纳米颗粒（DI-SeNPs），并通过紫外可见吸收光谱、透射电子显微镜、能谱分析、X射线衍射、zeta电位测量和傅里叶变换红外光谱对其进行了全面表征。DI-SeNPs对人肺腺癌细胞系（A549；半数抑制浓度为43.21μg/mL）表现出剂量依赖性毒性。DI-SeNPs增加了A549细胞中活性氧（ROS）的生成。DI-SeNPs导致A549细胞在G2/M期发生细胞周期阻滞，并增加了DNA损伤，最终促使这些细胞走向凋亡。DI-SeNPs显著提高了A549细胞中p53水平，降低了Akt水平，并提高了裂解的半胱天冬酶3水平。此外，DI-SeNPs对多种细菌和真菌表现出抗菌活性。这些发现表明，DI-SeNPs具有显著的抗癌和抗菌特性，其作用机制涉及ROS生成、细胞周期阻滞和凋亡诱导。
