Mirhashemi Majid, Saghravanian Nasrollah, Ghazi Narges, Abdoljavadi Aref
Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Indian J Otolaryngol Head Neck Surg. 2024 Dec;76(6):5478-5485. doi: 10.1007/s12070-024-05008-9. Epub 2024 Aug 30.
Oral squamous cell carcinoma (OSCC) comprises more than 90% of oral cavity cancer and remains the leading cause of death in oral disease. Limited studies have been conducted to evaluate cellular histomorphometry changes in OSCC compared to premalignant lesions such as Dysplastic leukoplakia (DL), Nondysplastic leukoplakia (NDL), and normal epithelial. This cross-sectional descriptive-analytical study was conducted on total 72 samples, including superficial areas of squamous cell carcinoma (SCCSF), Invasive Front of Squamous Cell Carcinoma (SCCIF), Apparently Normal Adjacent Oral Mucosa (SCCANM) or normal margin, Dysplastic leukoplakia (DL), Nondysplastic leukoplakia (NDL), and normal oral mucosa tissue (NOM) ( = 12 per group). ANOVA was used to compare the nucleus-to-cytoplasm ratio (N/C), nucleus area (NA), and cellular area (CA) of the stained hematoxylin and eosin (H&E) samples in the studied groups. A value less than 0.05 was considered to be a significant level. There was a significant increase in the CA, NA, and N/C in the basal and parabasal layers from normal epithelium to dysplastic epithelium and OSCC. The highest NA, CA, and N/C were in the SCCIF and SCCSF groups, respectively, and the lowest was observed in NOM. In addition, SCCANM basal and parabasal layer cells had a significant difference in N/C compared to NOM, which indicates a high risk of SCCANM transformation into malignancy. Cell histomorphometry changes were observed from normal tissue to premalignant lesions and OSCC. These parameters can be used as indicators of the potential for transformation into malignancy in premalignant lesions.
口腔鳞状细胞癌（OSCC）占口腔癌的90%以上，仍然是口腔疾病的主要死因。与发育异常的白斑（DL）、非发育异常的白斑（NDL）和正常上皮等癌前病变相比，评估OSCC细胞组织形态计量学变化的研究有限。本横断面描述性分析研究共对72个样本进行了检测，包括鳞状细胞癌浅表区域（SCCSF）、鳞状细胞癌浸润前沿（SCCIF）、明显正常的相邻口腔黏膜（SCCANM）或正常边缘、发育异常的白斑（DL）、非发育异常的白斑（NDL）以及正常口腔黏膜组织（NOM）（每组 = 12个）。采用方差分析比较研究组中苏木精和伊红（H&E）染色样本的核质比（N/C）、核面积（NA）和细胞面积（CA）。P值小于0.05被认为具有显著意义。从正常上皮到发育异常上皮和OSCC，基底层和副基底层的CA、NA和N/C均显著增加。NA、CA和N/C最高的分别是SCCIF组和SCCSF组，最低的是NOM组。此外，与NOM相比，SCCANM基底层和副基底层细胞的N/C有显著差异，这表明SCCANM恶变风险较高。从正常组织到癌前病变再到OSCC均观察到细胞组织形态计量学变化。这些参数可作为癌前病变恶变潜能的指标。
