Ma Chunqiu, Xu Jichao, Wang Xiaolu, Wang Xuewen, Zhang Lei, Jing Su
School of Chemistry and Molecular Engineering, Nanjing Tech University 30 South Puzhu Road Nanjing 211816 Jiangsu China
RSC Adv. 2024 Nov 18;14(49):36754-36762. doi: 10.1039/d4ra05923a. eCollection 2024 Nov 11.
We report the development of a diglycosyldiselenide-based fluorescent probe for the rapid detection of sulfhydryl-containing biomolecules. The probe facilitates a chemoselective coupling reaction with sulfhydryl groups in aqueous buffer under ambient conditions, resulting in the formation of homogeneous Se-S conjugates within one hour. Using glutathione, a sulfhydryl-containing biomolecule, as a proof of concept, the probe achieved a detection limit of 0.75 μM based on the 3 criterion. The method was further extended to the fluorescent labeling of cysteine-containing peptides, proteins, and living bacterial cells, showcasing the utility of Se-S covalent-directed chemistry as an analytical tool. This approach underscores the considerable potential of diglycosyldiselenide-based fluorescent probes for broader applications in biochemical research.
我们报道了一种基于二糖基二硒化物的荧光探针的开发，用于快速检测含巯基的生物分子。该探针在环境条件下于水性缓冲液中促进与巯基的化学选择性偶联反应，一小时内形成均一的硒 - 硫缀合物。以含巯基生物分子谷胱甘肽作为概念验证，该探针基于3准则实现了0.75 μM的检测限。该方法进一步扩展到含半胱氨酸的肽、蛋白质和活细菌细胞的荧光标记，展示了硒 - 硫共价导向化学作为一种分析工具的实用性。这种方法强调了基于二糖基二硒化物的荧光探针在生物化学研究中更广泛应用的巨大潜力。
