Zhang Hongyan, Pan Yue, Jiang Ying, Chen Mengling, Ma Xin, Yu Xueping, Ren Dayong, Jiang Bin
College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin Province, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
NPJ Sci Food. 2024 Nov 19;8(1):97. doi: 10.1038/s41538-024-00339-x.
Akermansia muciniphila shows promise as a next-generation probiotic, however, its beneficial regulatory effects on mice ulcerative colitis (UC) has not been extensively investigated. We used an Akkermansia muciniphila strain (AKK ONE) isolated from healthy human feces to study its effect on DSS-induced colitis in mice. Our results demonstrate that AKK ONE supplementation significantly improves food intake, weight, colon length, disease activity index (DAI) score, organ index, and tissue damage of colitis mice. AKK ONE notably improved intestinal barrier integrity by significantly enhancing expression of occludin and claudin-1. Additionally, AKK ONE reduced inflammation by down-regulating IL-1β, IL-6, and TNF-α, and up-regulating IL-10. In addition to reducing excessive inflammation, AKK ONE also increased the abundance of Akkermansia and decreased the abundance of Bacteroides. Furthermore, the AKK ONE intervention markedly increased SCFAs in cecal contents. AKK ONE may be a potential therapeutic agent for improving UC, based on the findings of this study.
嗜黏蛋白阿克曼氏菌有望成为下一代益生菌，然而，其对小鼠溃疡性结肠炎（UC）的有益调节作用尚未得到广泛研究。我们使用从健康人粪便中分离出的一株嗜黏蛋白阿克曼氏菌（AKK ONE）来研究其对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的影响。我们的结果表明，补充AKK ONE可显著改善结肠炎小鼠的食物摄入量、体重、结肠长度、疾病活动指数（DAI）评分、器官指数和组织损伤。AKK ONE通过显著增强闭合蛋白和紧密连接蛋白-1的表达，显著改善了肠道屏障完整性。此外，AKK ONE通过下调白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α以及上调白细胞介素-10来减轻炎症。除了减轻过度炎症外，AKK ONE还增加了阿克曼氏菌的丰度并降低了拟杆菌的丰度。此外，AKK ONE干预显著增加了盲肠内容物中的短链脂肪酸（SCFA）。基于本研究的结果，AKK ONE可能是改善UC的一种潜在治疗剂。
