Li Yang, Wang Shuai, Zhu Xuran, Gao Nana, Kang Jungang, Wang Tianxiong, Wang Xiaodan
College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding, China.
Chengde Academy of Agricultural and Forestry Sciences, Chengde, Hebei, China.
Front Microbiol. 2025 May 27;16:1570370. doi: 10.3389/fmicb.2025.1570370. eCollection 2025.
polysaccharides (PLP) have been shown to have beneficial effects on gut function and gut microbiota in animals. The intention of this study was to examine the effects of PLP on growth performance, immune function, intestinal barrier function and intestinal microbial community in broilers under lipopolysaccharide (LPS) challenge. A total of 120 one-day-old Arbor Acres (AA) broilers were randomly allocated into 3 groups: control, LPS, and PLP + LPS. The PLP + LPS group had 200 mg/kg/d PLP added to the ration in the daily trial. LPS and PLP + LPS group were intraperitoneally injected with LPS on days 14, 16, 18, and 20. The results demonstrated that LPS significantly decreased average daily gain (ADG), average daily feed intake (ADFI), and total antioxidant capacity (T-AOC) activity in serum and duodenum. Additionally, LPS reduced the mRNA expression levels of and in duodenum, increased spleen bursal index, and MDA activity in serum and duodenum ( < 0.05). Histological examination revealed that LPS caused duodenal damage, leading to intestinal mucosal injury and shedding, villus height reduction, and crypt depth increase. PLP inclusion mitigated the adverse impacts of LPS on key parameters ( < 0.05). Furthermore, gut microbiota analysis revealed that PLP supplementation increased the ratio of to . At the genus level, the addition of PLP decreased the abundance of and , while simultaneously increased the presence of , and . In summary, supplementing the dietary inclusion with 200 mg/kg of PLP enhances growth, immune function, and antioxidant capabilities in LPS-challenged broilers. This improvement is likely attributed to the modulation of gut microbiota community composition.
多糖（PLP）已被证明对动物的肠道功能和肠道微生物群具有有益作用。本研究的目的是探讨PLP对脂多糖（LPS）攻击下肉鸡生长性能、免疫功能、肠道屏障功能和肠道微生物群落的影响。总共120只1日龄的艾维茵（AA）肉鸡被随机分为3组：对照组、LPS组和PLP + LPS组。在每日试验中，PLP + LPS组在日粮中添加200 mg/kg/d的PLP。LPS组和PLP + LPS组在第14、16、18和20天腹腔注射LPS。结果表明，LPS显著降低了血清和十二指肠中的平均日增重（ADG）、平均日采食量（ADFI）和总抗氧化能力（T-AOC）活性。此外，LPS降低了十二指肠中 和 的mRNA表达水平，增加了脾脏法氏囊指数以及血清和十二指肠中的丙二醛（MDA）活性（P < 0.05）。组织学检查显示，LPS导致十二指肠损伤，导致肠黏膜损伤和脱落、绒毛高度降低和隐窝深度增加。添加PLP减轻了LPS对关键参数的不利影响（P < 0.05）。此外，肠道微生物群分析显示，补充PLP增加了 与 的比例。在属水平上，添加PLP降低了 和 的丰度，同时增加了 、 和 的存在。总之，在日粮中添加200 mg/kg的PLP可提高LPS攻击下肉鸡的生长、免疫功能和抗氧化能力。这种改善可能归因于肠道微生物群落组成的调节。
