Pan-genomic characterization and structural variant analysis reveal insights into spore development and species diversity in .

Understanding the genomic diversity and functional implications of species is crucial for elucidating their evolutionary history and biotechnological potential. Here, we present the first pan-genomic analysis of spp., combining five newly sequenced genomes with ten publicly available genomes. Our comprehensive comparative study unveiled a rich genomic landscape, identifying core genes shared among all strains and species-specific gene sets. Additionally, we identified structural variants impacting the expression of key genes, including insights into the gene involved in DNA repair and recombination processes, which exhibits a 440 bp insertion in the promoter region and a leucine-to-serine mutation in the gene body, potentially increasing spore production in the S3 strain. Overall, our study provides valuable insights into the genomic architecture and functional diversity of , paving the way for further research on its evolutionary dynamics, biotechnological applications and pharmaceutical potential.