Su Xiumin, Su Li, Cao Mengyuan, Sun Yulu, Dai Jinghan, He Yuanjie, Li Wei, Ge Wupeng, Lv Xin, Zhang Qiang, Cui Shenghui, Chen Jia, Yang Baowei
College of Food Science and Engineering, Northwest A&F University, 28# Xinong Road, Yangling 712100, Xianyang, Shaanxi, China.
College of Life Science, Northwest A&F University, Yangling 712100, Xianyang, China.
Probiotics Antimicrob Proteins. 2024 Nov 20. doi: 10.1007/s12602-024-10401-5.
In this study, four lactic acid bacteria (LAB) strains demonstrating ciprofloxacin, bile salt, gastric fluid, and intestinal fluid tolerance as well as adhesion ability to Caco-2 and HT-29 cells were used to improve and recover the intestinal flora disorders caused by ciprofloxacin, among which, Lactobacillus brevis 505 exhibited excellent adhesion ability to two kinds of cells and colonization ability to mouse intestinal. After ciprofloxacin treatment, certain recovery effect on cecum caused by ciprofloxacin in the mice was found during natural recovery (group 5C2), but it was challenging to fully restore the intestinal integrity to the initial level. After L. brevis 505 intervention (group 5C5), the intestinal damage to the colon and ileum caused by ciprofloxacin in mice was significantly alleviated; the recovery effect was better than that of natural recovery. Additionally, L. brevis 505 could effectively regulate INF-γ, sIgA, and RegIIIγ increase induced by ciprofloxacin. Shannon and Simpson index of the intestinal flora of mice in 5C5 group were higher than those in other group, the relative abundance of Bifidobacterium and Lactobacillus in the mice in 5C5 group was increased, indicating that LAB can better restore the structure and abundance of intestinal microflora. Consequently, L. brevis 505 shows promise as a probiotic for gut microbiota restoration and rebuilding during antibiotic therapy.
在本研究中，使用了四株对环丙沙星、胆盐、胃液和肠液具有耐受性且对Caco-2和HT-29细胞具有黏附能力的乳酸菌（LAB）菌株，以改善和恢复由环丙沙星引起的肠道菌群紊乱，其中，短乳杆菌505对两种细胞表现出优异的黏附能力以及对小鼠肠道的定植能力。在环丙沙星处理后，在自然恢复过程中（5C2组）发现对小鼠盲肠由环丙沙星引起的损伤有一定恢复作用，但要将肠道完整性完全恢复到初始水平具有挑战性。在短乳杆菌505干预后（5C5组），环丙沙星对小鼠结肠和回肠造成的肠道损伤明显减轻；恢复效果优于自然恢复。此外，短乳杆菌505可以有效调节由环丙沙星诱导的INF-γ、sIgA和RegIIIγ的增加。5C5组小鼠肠道菌群的香农指数和辛普森指数高于其他组，5C5组小鼠中双歧杆菌和乳酸菌的相对丰度增加，表明乳酸菌能够更好地恢复肠道微生物群的结构和丰度。因此，短乳杆菌505有望作为一种益生菌用于抗生素治疗期间肠道微生物群的恢复和重建。
