Groves D J, Batten T F
Gen Comp Endocrinol. 1986 Mar;61(3):402-16. doi: 10.1016/0016-6480(86)90226-1.
Pituitaries from male and female fish were incubated with 1, 5, and 10 micrograms/ml of oestradiol-17 beta, testosterone, 17 alpha OH-progesterone, and 17 alpha,20 beta-dihydroxy-4-pregnan-3-one for 18 hr. Ultrastructural differences between control and experimentally treated glands were used to define the direct effects of these steroids on the gonadotropic cells of the adenohypophysis. The effects of the steroids differed according to sex and reproductive state of the donor animal. Oestradiol and testosterone stimulated gonadotropin secretion by active gonadotrophs, but inhibited it in inactive cells. Both the progesterones generally inhibited gonadotropin secretion although 17 alpha,20 beta-dihydroxy-4-pregnan-3-one had no effect on active gonadotrophs. The four steroids investigated all show potential for direct control of gonadotropin secretion in Poecilia latipinna although factors affecting the balance of these actions, and their relative importance in vivo, remain to be elucidated.
将雄性和雌性鱼类的脑垂体分别与浓度为1、5和10微克/毫升的17β-雌二醇、睾酮、17α-羟基孕酮以及17α,20β-二羟基-4-孕烯-3-酮一起孵育18小时。通过对照腺体与经实验处理的腺体之间的超微结构差异来确定这些类固醇对腺垂体促性腺细胞的直接作用。类固醇的作用因供体动物的性别和生殖状态而异。雌二醇和睾酮刺激活跃的促性腺细胞分泌促性腺激素，但抑制不活跃细胞的分泌。两种孕酮通常都抑制促性腺激素的分泌，尽管17α,20β-二羟基-4-孕烯-3-酮对活跃的促性腺细胞没有影响。所研究的四种类固醇均显示出直接控制食蚊鱼促性腺激素分泌的潜力，不过影响这些作用平衡的因素及其在体内的相对重要性仍有待阐明。
