Sharma Aakash, Singh Amritpal, Pendyala Brahmaiah, Balamurugan Sampathkumar, Patras Ankit
Department of Food and Animal Sciences, Tennessee State University, Nashville, Tennessee, USA.
Guelph Research and Development Centre, Agriculture and Agri-Food Canada Guelph, Guelph, Canada.
Appl Environ Microbiol. 2025 Jan 31;91(1):e0109324. doi: 10.1128/aem.01093-24. Epub 2024 Nov 21.
The airborne transmission of infectious diseases and bioaerosol-induced cross-contamination pose significant challenges in the food, dairy, and pharma industries. This study evaluated the effectiveness of 279 nm UV-C LED irradiation for decontaminating bioaerosols, specifically containing microorganisms such as (C3040- Kanamycin resistant), Enteritidis (ATCC 4931), and (ATCC 4973), on food contact surfaces. Borosilicate glass, silicon rubber, and stainless steel (316L) surfaces were selected for experimentation for their usage in the food industry. A 50 µL cell suspension was aerosolized at 25 psi pressure using a 4-jet BLAM Nebulizer within a customized glass chamber and then deposited onto the surface of the coupons. The serial dilution approach was used for the microbial enumeration, followed by duplicate plating. With a low Root Mean Square Error (RMSE) and high values, the biphasic kinetic model for UV-C inactivation curves of all three pathogens demonstrated the excellent goodness of fit parameters. At a UV-C dose of 6 mJ cm, glass surfaces showed the maximum microbial inactivation (i.e., 2.80, 3.81, and 3.56 log CFU/mL for , , and , respectively). Stainless steel and silicon rubber surfaces showed significant microbial inactivation, but log reductions observed were consistently lower than glass surface. Our research indicates that UV-C LEDs (279 nm) can effectively disinfect bioaerosols on food contact surfaces.IMPORTANCEFood safety is a major public health concern, with contaminated food causing serious illnesses. UV-C light, used for germicidal action, is effective in disinfecting surfaces and is not subject to the same strict legal restrictions as chemical disinfectants, simplifying compliance with food safety regulations. In this study, we evaluated the efficacy of UV-C (279 nm) LED systems for inactivation of surface-deposited bioaerosols of kanamycin-resistant (C3040), Enteritidis (ATCC 4931), and (ATCC 4973). The research outcomes can be used to develop UV-based surface disinfection systems to minimize the risk of foodborne illnesses and enhance safety in high-traffic food preparation areas.
传染病的空气传播以及生物气溶胶引起的交叉污染给食品、乳制品和制药行业带来了重大挑战。本研究评估了279纳米紫外线C发光二极管（UV-C LED）照射对食品接触表面上生物气溶胶的去污效果，这些生物气溶胶具体含有诸如（C3040 - 耐卡那霉素）、肠炎沙门氏菌（ATCC 4931）和（ATCC 4973）等微生物。选择硼硅酸盐玻璃、硅橡胶和不锈钢（316L）表面进行实验，因为它们在食品工业中有应用。使用4喷头BLAM雾化器在定制玻璃室内以25磅力/平方英寸的压力将50微升细胞悬液雾化，然后沉积在试片表面。采用系列稀释法进行微生物计数，随后进行重复平板接种。所有三种病原体的UV-C灭活曲线的双相动力学模型具有较低的均方根误差（RMSE）和较高的值，表明拟合参数的拟合优度极佳。在UV-C剂量为6毫焦/平方厘米时，玻璃表面显示出最大的微生物灭活效果（即对于、和，分别为2.80、3.81和3.56对数CFU/毫升）。不锈钢和硅橡胶表面显示出显著的微生物灭活效果，但观察到的对数减少始终低于玻璃表面。我们的研究表明，UV-C LED（279纳米）可有效消毒食品接触表面上的生物气溶胶。重要性食品安全是一个主要的公共卫生问题，受污染的食品会导致严重疾病。用于杀菌作用的UV-C光在表面消毒方面有效，并且不像化学消毒剂那样受到同样严格的法律限制，简化了食品安全法规的合规性。在本研究中，我们评估了UV-C（279纳米）LED系统对表面沉积的耐卡那霉素（C3040）、肠炎沙门氏菌（ATCC 4931）和（ATCC 4973）生物气溶胶的灭活效果。研究结果可用于开发基于紫外线的表面消毒系统，以最大限度地降低食源性疾病的风险，并提高高流量食品制备区域的安全性。
