Polen Breanna, Patras Ankit, Pendyala Brahmaiah, D'Souza Doris H
Department of Food Science, University of Tennessee, Knoxville, TN 37996, USA.
Department of Food and Animal Sciences, Tennessee State University, Nashville, TN 37209, USA.
Foods. 2025 May 27;14(11):1899. doi: 10.3390/foods14111899.
Hepatitis A viral outbreaks continue to occur. It can be transmitted through aerosolized droplets and thus can contaminate surfaces and the environment. Ultraviolet light emitting diode (UV-C LED) systems are used for inactivation of microbes, though research is needed to determine optimal doses for aerosolized HAV inactivation. This study evaluates the UV-C LED doses for the inactivation of aerosolized hepatitis A virus (HAV) deposited on stainless-steel and glass discs. HAV was aseptically deposited onto stainless-steel or glass discs (1.27 cm diameter) using a nebulizer within a chamber followed by treatments for up to 1.5 min with 255 nm (surface dose = 0-76.5 mJ/cm) or 279 nm (surface dose = 0-8.1 mJ/cm) UV-C LED. Plaque assays were used to enumerate infectious titers of recovered viruses and data from three replicates were statistically analyzed. The calculated linear D-value (UV-C dose for a 1-log reduction in aerosolized deposits) for HAV by 255 nm UV-C LED was 47.39 ± 7.40 and 40.0 ± 2.94 mJ/cm (R = 0.94 and 0.91) and using 279 nm UV-C LED were 6.60 ± 0.27 and 5.57 ± 0.74 mJ/cm (R = 0.98 and 0.94) on stainless-steel and glass discs, respectively. The non-linear Weibull model showed δ (dose needed for a 1-log reduction in aerosolized HAV deposits) values for HAV of 29.69 ± 5.49 and 35.25 ± 15.01 mJ/cm by 255 nm UV-C LED (R = 0.99 and 0.92) and 6.67 ± 0.63 and 5.21 ± 1.25 mJ/cm by 279 nm UV-C LED (R = 0.98 and 0.95) on stainless-steel and glass discs, respectively. These data indicate that 279 nm UV-C LED showed higher efficiency for HAV inactivation than 255 nm UV-C LED, and that Weibull models were a better fit when tailing was observed. This study provides the inactivation data needed to aid in designing UV-C LED systems for delivering doses required to inactivate bio-aerosolized HAV deposits on stainless-steel and glass.
甲型肝炎病毒疫情仍时有发生。它可通过气溶胶飞沫传播，进而污染表面和环境。紫外线发光二极管（UV-C LED）系统用于微生物灭活，不过仍需开展研究以确定气溶胶化甲型肝炎病毒灭活的最佳剂量。本研究评估了UV-C LED剂量对沉积在不锈钢和玻璃圆盘上的气溶胶化甲型肝炎病毒（HAV）的灭活效果。在一个腔室内，使用雾化器将HAV无菌沉积到不锈钢或玻璃圆盘（直径1.27厘米）上，然后用255纳米（表面剂量 = 0 - 76.5毫焦/平方厘米）或279纳米（表面剂量 = 0 - 8.1毫焦/平方厘米）的UV-C LED进行长达1.5分钟的处理。采用噬斑测定法对回收病毒的感染滴度进行计数，并对来自三个重复实验的数据进行统计分析。255纳米UV-C LED对不锈钢圆盘和玻璃圆盘上HAV的计算线性D值（使气溶胶化沉积物减少1个对数级的UV-C剂量）分别为47.39 ± 7.40和40.0 ± 2.94毫焦/平方厘米（R = 0.94和0.91），279纳米UV-C LED的分别为6.60 ± 0.27和5.57 ± 0.74毫焦/平方厘米（R = 0.98和0.94）。非线性韦布尔模型显示，255纳米UV-C LED对不锈钢圆盘和玻璃圆盘上HAV的δ值（使气溶胶化HAV沉积物减少1个对数级所需的剂量）分别为29.69 ± 5.
