Universidade Federal de São João Del-Rei, Programas de Pós-graduação em Biotecnologia e Multicêntrico em Bioquímica e Biologia Molecular, Disciplina Biotecnologia & Inovações, Divinópolis, MG, Brasil.
Universidade Federal de São João Del-Rei, Laboratório de Biotecnologia de Microrganismos, Instituto Nacional de Ciência e Tecnologia em Biotecnologia Industrial, Divinópolis, MG, Brasil.
Rev Soc Bras Med Trop. 2024 Nov 15;57. doi: 10.1590/0037-8682-0226-2024. eCollection 2024.
Rickettsiosis is a disease caused by bacteria belonging to the genus Rickettsia, and is a potentially fatal zoonotic disease of great medical and veterinary importance. Given the urgent need to develop new diagnostic methods for detecting this disease, the present review aimed to evaluate the number of publications dedicated to the identification of Rickettsia spp. in human samples using molecular methods, such as polymerase chain reaction and its variations. To this end, a bibliographical survey covering articles published in the past ten years was conducted using the PudMed platform with the keywords "spotted fever" and "Rickettsia," both combined with "diagnosis." A growing number of publications in this area reflects an increasing interest in research, especially since 2015. From 2015 to February 2024, several promising results were tested and many studies were able to detect the genetic sequences of interest. Therefore, the absence of a standard diagnosis method highlights the critical need for developing an effective technique capable of accurately detecting the etiological agent and ensuring accurate diagnosis of the disease.
立克次体病是由立克次体属细菌引起的疾病，是一种具有重要医学和兽医学意义的潜在致命人畜共患病。鉴于迫切需要开发新的诊断方法来检测这种疾病，本综述旨在评估过去十年中使用聚合酶链反应及其变体等分子方法鉴定人类样本中立克次体属的研究数量。为此，使用 PudMed 平台，使用“斑疹热”和“立克次体”这两个关键词，以及“诊断”，对过去十年发表的文章进行了文献调查。该领域越来越多的出版物反映了人们对此项研究的兴趣日益浓厚，尤其是自 2015 年以来。从 2015 年到 2024 年 2 月，已经测试了许多有前途的结果，并且许多研究能够检测到感兴趣的遗传序列。因此，缺乏标准诊断方法突出表明迫切需要开发一种有效的技术，能够准确检测病因，并确保对疾病的准确诊断。
